Tissue patrol by resident memory CD8+ T cells in human skin

Emerging data show that tissue-resident memory T (T RM ) cells play an important protective role at murine and human barrier sites. T RM cells in the epidermis of mouse skin patrol their surroundings and rapidly respond when antigens are encountered. However, whether a similar migratory behavior is...

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Bibliographic Details
Published in:Nature immunology Vol. 20; no. 6; pp. 756 - 764
Main Authors: Dijkgraaf, Feline E., Matos, Tiago R., Hoogenboezem, Mark, Toebes, Mireille, Vredevoogd, David W., Mertz, Marjolijn, van den Broek, Bram, Song, Ji-Ying, Teunissen, Marcel B. M., Luiten, Rosalie M., Beltman, Joost B., Schumacher, Ton N.
Format: Journal Article
Language:English
Published: New York Nature Publishing Group US 01.06.2019
Nature Publishing Group
Subjects:
631/250/1619/554/1834
631/250/2152/1566/1571
631/250/2503
631/80/84
Animal models
Animals
Antigens
Antigens - immunology
Biomedical and Life Sciences
Biomedicine
CD8 antigen
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - metabolism
Cell culture
Cell Line, Tumor
Cell Movement - immunology
Dermis
Epidermis
Epidermis - immunology
Epidermis - metabolism
Fluorescent Antibody Technique
Humans
Immunologic Memory
Immunological memory
Immunology
Infectious Diseases
Langerhans cells
Lymphocytes T
Memory cells
Mice
Nanobodies
Organ Specificity - immunology
Single-Domain Antibodies - immunology
Skin
Skin - immunology
Skin - metabolism
technical-report
Vaccines, DNA - genetics
Vaccines, DNA - immunology
ISSN:1529-2908, 1529-2916, 1529-2916
Online Access:Get full text
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Summary:Emerging data show that tissue-resident memory T (T RM ) cells play an important protective role at murine and human barrier sites. T RM cells in the epidermis of mouse skin patrol their surroundings and rapidly respond when antigens are encountered. However, whether a similar migratory behavior is performed by human T RM cells is unclear, as technology to longitudinally follow them in situ has been lacking. To address this issue, we developed an ex vivo culture system to label and track T cells in fresh skin samples. We validated this system by comparing in vivo and ex vivo properties of murine T RM cells. Using nanobody labeling, we subsequently demonstrated in human ex vivo skin that CD8 + T RM cells migrated through the papillary dermis and the epidermis, below sessile Langerhans cells. Collectively, this work allows the dynamic study of resident immune cells in human skin and provides evidence of tissue patrol by human CD8 + T RM cells. Tissue-resident memory T (T RM ) cells have been studied mainly in mouse models. Schumacher and colleagues have developed an imaging technology to track in real time skin-resident human CD8 + T RM cells in situ.
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ISSN:1529-2908
1529-2916
1529-2916
DOI:10.1038/s41590-019-0404-3