Fabrication of chondroitin sulfate calcium complex and its chondrocyte proliferation in vitro

[Display omitted] •Chondroitin sulfate calcium complex (CSCa) was fabricated by cation exchange method.•Characterization of CSCa was analyzed by EDS, AFM, FTIR, NMR, XRD, TGA and DSC.•Chondrocyte proliferation bioactivity of CSCa was investigated in vitro.•CSCa increased intracellular calcium ions a...

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Published in:Carbohydrate polymers Vol. 254; p. 117282
Main Authors: Shen, Qingshan, Zhang, Chunhui, Mo, Haizhen, Zhang, Hongru, Qin, Xiaojie, Li, Juan, Zhang, Zhiqiang, Richel, Aurore
Format: Journal Article
Language:English
Published: England Elsevier Ltd 15.02.2021
Subjects:
Apoptosis
Apoptosis - drug effects
bioactive properties
Bone care
Calcium
Calcium - chemistry
Calcium - metabolism
Calcium - pharmacology
calcium receptors
Calcium sensing receptors
Carboxyl groups
CASR protein, human
Cell Cycle
Cell Cycle - drug effects
Cell Proliferation
Cell Proliferation - drug effects
Cells, Cultured
Chemistry
Chimie
Chondrocyte
Chondrocyte proliferation
Chondrocytes
Chondrocytes - cytology
Chondrocytes - drug effects
Chondrocytes - metabolism
Chondroitin sulfate
Chondroitin Sulfates
Chondroitin Sulfates - chemical synthesis
Chondroitin Sulfates - chemistry
Chondroitin Sulfates - pharmacology
Food supplements
Gene Expression
Holding capacity
Humans
In Vitro Techniques
Intracellular calcium
Magnetic Resonance Spectroscopy
Materials Chemistry
Models, Biological
Molecular Docking Simulation
Molecular Structure
nuclear magnetic resonance spectroscopy
Organic Chemistry
Particle Size
Physical, chemical, mathematical & earth Sciences
Physique, chimie, mathématiques & sciences de la terre
Polymers and Plastics
Proliferation
Receptors, Calcium-Sensing
Receptors, Calcium-Sensing - chemistry
Receptors, Calcium-Sensing - genetics
Receptors, Calcium-Sensing - metabolism
secretion
Spectroscopy, Fourier Transform Infrared
Structural characteristics
sulfates
TGFB1 protein, human
thermal stability
Transforming Growth Factor beta1
Transforming Growth Factor beta1 - metabolism
Bone care
Chondroitin sulfate
Proliferation
Calcium
Chondrocyte
ISSN:0144-8617, 1879-1344, 1879-1344
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Summary:[Display omitted] •Chondroitin sulfate calcium complex (CSCa) was fabricated by cation exchange method.•Characterization of CSCa was analyzed by EDS, AFM, FTIR, NMR, XRD, TGA and DSC.•Chondrocyte proliferation bioactivity of CSCa was investigated in vitro.•CSCa increased intracellular calcium ions and activated the TGF-β/Smads pathway. Chondroitin sulfate (CS)-calcium complex (CSCa) was fabricated, and the structural characteristics of CSCa and its proliferative bioactivity to the chondrocyte were investigated in vitro. Results suggested calcium ions could bind CS chains forming polysaccharide-metal complex, and the maximum calcium holding capacity of CSCa reached 4.23 %. Characterization of CSCa was performed by EDS, AFM, FTIR, UV, XRD and 1H-NMR. It was found that calcium ions were integrated with CS by binding the sulfate or carboxyl groups. The thermal properties analysis indicated CSCa had a good thermal stability by TGA and DSC. CSCa could interact the calcium-sensing receptor increasing the intracellular calcium ions and influence the cell cycle. The TGF-β1 secretion induced by CSCa could activate the TGF-β/Smads pathway and change the genes associated proliferation expression ultimately leading to the chondrocyte proliferation. This research probably has an important implication for understanding the effect of CSCa on bone care as food supplements.
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scopus-id:2-s2.0-85094821033
ISSN:0144-8617
1879-1344
1879-1344
DOI:10.1016/j.carbpol.2020.117282