In silico rational design and systems engineering of disulfide bridges in the catalytic domain of an alkaline α-amylase from Alkalimonas amylolytica to improve thermostability

High thermostability is required for alkaline α-amylases to maintain high catalytic activity under the harsh conditions used in textile production. In this study, we attempted to improve the thermostability of an alkaline α-amylase from Alkalimonas amylolytica through in silico rational design and s...

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Veröffentlicht in:	Applied and environmental microbiology Jg. 80; H. 3; S. 798
Hauptverfasser:	Liu, Long, Deng, Zhuangmei, Yang, Haiquan, Li, Jianghua, Shin, Hyun-dong, Chen, Rachel R, Du, Guocheng, Chen, Jian
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.02.2014
Schlagworte:	alpha-Amylases - chemistry alpha-Amylases - genetics alpha-Amylases - metabolism Catalytic Domain Cysteine - genetics Cysteine - metabolism Disulfides - metabolism Enzyme Stability Gammaproteobacteria - enzymology Gammaproteobacteria - genetics Protein Engineering - methods Temperature
ISSN:	1098-5336, 1098-5336
Online-Zugang:	Weitere Angaben
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