The interaction of Pax5 (BSAP) with Daxx can result in transcriptional activation in B cells

Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. Using a yeast two-hybrid assay to identify potential coregulators of Pax5, we identified Daxx, a protein that is highly conserved, ubiquitously expressed, and essential for embryonic mouse d...

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Veröffentlicht in:The Journal of biological chemistry Jg. 277; H. 13; S. 11156
Hauptverfasser: Emelyanov, Alexander V, Kovac, Cecilia R, Sepulveda, Manuel A, Birshtein, Barbara K
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 29.03.2002
Schlagworte:
Adaptor Proteins, Signal Transducing
Animals
B-Lymphocytes - cytology
B-Lymphocytes - metabolism
Base Sequence
Carrier Proteins - metabolism
Cell Differentiation
Cell Line
Co-Repressor Proteins
DNA Primers
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
Humans
Intracellular Signaling Peptides and Proteins
Mice
Molecular Chaperones
Nuclear Proteins
PAX5 Transcription Factor
Transcription Factors - chemistry
Transcription Factors - metabolism
Transcriptional Activation
ISSN:0021-9258
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Zusammenfassung:Pax5 (BSAP) is essential for B cell development and acts both as a transcriptional activator and a repressor. Using a yeast two-hybrid assay to identify potential coregulators of Pax5, we identified Daxx, a protein that is highly conserved, ubiquitously expressed, and essential for embryonic mouse development. The interaction between Pax5 and Daxx involves the partial homeodomain of Pax5 and the C-terminal fragment of Daxx. A component of promyelocytic leukemia protein nuclear bodies, Daxx has been implicated in apoptosis and characterized as a transcriptional corepressor. Upon transient transfection assay of Daxx in B cells expressing endogenous Daxx and Pax5, we observed not only transcriptional corepression but also, unexpectedly, coactivation in M12.4.1 and A20 mouse B cell lines. Pax5 domains required for coactivation were identified using 293T cells. Coactivation apparently involves recruitment of the CREB binding protein (CBP), because we precipitated complexes containing Pax5, Daxx, and CBP in B cell lines. These data suggest that Daxx can affect Pax5's roles as an activator or repressor in B cells and describe a role for Daxx as a transcriptional coactivator.
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ISSN:0021-9258
DOI:10.1074/jbc.M111763200