Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: An in vitro study

Eclipta alba is traditionally used as hepatoprotective agent. The study was designed to explore its antiproliferative activity on liver and other related cancer. The present study was designed to assess and establish the role of Eclipta alba as anti-cancer agent using HepG2, C6 glioma and A498 cell...

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Veröffentlicht in:Journal of ethnopharmacology Jg. 136; H. 2; S. 363 - 367
Hauptverfasser: Chaudhary, Harshita, Dhuna, Vikram, Singh, Jatinder, Kamboj, Sukhdev Singh, Seshadri, Sriram
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Shannon Elsevier B.V 22.06.2011
Elsevier Ireland Ltd
Elsevier
Schlagworte:
Anti-metastasis
Antineoplastic Agents, Phytogenic
Antineoplastic Agents, Phytogenic - pharmacology
Antineoplastic Agents, Phytogenic - therapeutic use
apoptosis
Apoptosis - drug effects
Apoptotic
Biological and medical sciences
Cell Line, Tumor
cell proliferation
Cell Proliferation - drug effects
cytotoxicity
DNA Damage
Down-Regulation
drug effects
drug therapy
Eclipta
Eclipta alba
Eclipta prostrata
gelatin
General pharmacology
Glioma
Glioma - drug therapy
Glioma - metabolism
Hep G2 Cells
Hepatoblastoma
Hepatoblastoma - drug therapy
Hepatoblastoma - metabolism
Humans
in vitro studies
Inhibitory Concentration 50
liver
Liver Neoplasms
Liver Neoplasms - drug therapy
Liver Neoplasms - metabolism
Matrix Metalloproteinases
Matrix Metalloproteinases - metabolism
Medical sciences
metabolism
metalloproteinases
Neoplasms
Neoplasms - drug therapy
Neoplasms - metabolism
NF-kappa B
NF-kappa B - metabolism
NF-kB
Pharmacognosy. Homeopathy. Health food
pharmacology
Pharmacology. Drug treatments
Phytotherapy
Plant Extracts
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Plant Leaves
polymerase chain reaction
therapeutic use
Western blotting
Matrix metalloproteinases
Anti-metastasis
Eclipta alba
Apoptotic
NF-kB
Antineoplastic agent
Evaluation
Enzyme
Pharmacognosy
Metalloendopeptidases
Extract
Malignant tumor
Metastasis
In vitro
Medicinal plant
Peptidases
Plant origin
Hydrolases
Cancer
ISSN:0378-8741, 1872-7573, 1872-7573
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Zusammenfassung:Eclipta alba is traditionally used as hepatoprotective agent. The study was designed to explore its antiproliferative activity on liver and other related cancer. The present study was designed to assess and establish the role of Eclipta alba as anti-cancer agent using HepG2, C6 glioma and A498 cell lines as model system. Antiproliferative and cytotoxic effects of the Eclipta alba hydroalcoholic extract (EAE) was determined using MTT assay. The expression level of NF-kB was analysed by western blotting and RT PCR. Gelatin zymography was done for gelatinase matrix metalloproteinases (MMP-2 and 9) analysis. EAE inhibited the cell proliferation in dose dependent manner in HepG2, A498 and C6 glioma cell lines with an IC50 of 22±2.9, 25±3.6 and 50±8.7μg/ml, respectively. The expression of MMP (2 and 9) was down-regulated with EAE treatment. DNA damage was observed following 72h of extract treatment, leading to apoptosis. Additionally, the expression level of NF-kB was evaluated with western blotting and RT-PCR and was found to be down-regulated/inactivated. The data establish the existence of anti-proliferative, DNA damaging and anti-metastasis properties in EAE which is yet unexplored and hold high therapeutic impact.
Bibliographie:http://dx.doi.org/10.1016/j.jep.2011.04.066
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-8741
1872-7573
1872-7573
DOI:10.1016/j.jep.2011.04.066