Up-regulation of human immune system function by Donkey’s Milk

Donkey’s milk represents a good alternative to human milk because of its chemical characteristics similar to those of human’s. In present study, the pro- and anti-inflammatory effects of donkey’s milk were evaluated on peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from 12 young and...

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Bibliographic Details
Published in:Brazilian Journal of Pharmaceutical Sciences Vol. 56
Main Authors: Taghiloo, Saeid, Allahmoradi, Esmaeil, Sadeghian-Kiadehi, Seyedeh Forough, Omrani-Nava, Versa, Nazar, Eisa, Ebrahimzadeh, Mohammad Ali
Format: Journal Article
Language:English
Published: Sao Paulo Universidade de Sao Paulo Faculdade de Ciencias 01.01.2020
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Universidade de São Paulo
Subjects:
Aged subject
Blood
Breastfeeding & lactation
Cytokine
Cytokines
Donkey’s milk
ELISA
Enzymes
Immune system
Males
Milk
Natural products
PHARMACOLOGY & PHARMACY
Tumor necrosis factor-TNF
France
United States > US
California
Aged subject
Donkey’s milk
Cytokine
ELISA
Immune system
ISSN:2175-9790, 1984-8250, 2175-9790
Online Access:Get full text
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Summary:Donkey’s milk represents a good alternative to human milk because of its chemical characteristics similar to those of human’s. In present study, the pro- and anti-inflammatory effects of donkey’s milk were evaluated on peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from 12 young and 12 aged normal subjects. PBMCs were cultured with or without the optimal and non-cytotoxic dose of pasteurized donkey’s milk, and polymyxin B was used to inhibit the possible endotoxin contamination. Following 18 hours incubation, culture supernatants were harvested to measure the secreted Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Interleukin-10 (IL-10) by ELISA. Donkey’s milk significantly increased TNF-α (p= 0.01), IL-8 (p< 0.0001), IL-6 (p< 0.0001) and IL-10 (p= 0.01) levels in PBMCs. In addition, the levels of IL-6 (p= 0.002), IL-8 (p= 0.002) and TNF-α (p= 0.002) from aged subjects were significantly higher compared with young subjects. In contrast with these data, the level of IL-10 was markedly reduced from aged subjects (p= 0.02). Considering the immune-potentiation effects of donkey’s milk, it is suggested investigating milk as a beneficial dietary component for up-regulating the immune response in aged people.
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ISSN:2175-9790
1984-8250
2175-9790
DOI:10.1590/s2175-97902019000418449