Novel intravital approaches to quantify deep vascular structure and perfusion in the aging mouse brain using ultrasound localization microscopy (ULM)

Intra-vital visualization of deep cerebrovascular structures and blood flow in the aging brain has been a difficult challenge in the field of neurovascular research, especially when considering the key role played by the cerebrovasculature in the pathogenesis of both vascular cognitive impairment an...

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Veröffentlicht in:Journal of cerebral blood flow and metabolism Jg. 44; H. 11; S. 1378
Hauptverfasser: Nyúl-Tóth, Ádám, Negri, Sharon, Sanford, Madison, Jiang, Raymond, Patai, Roland, Budda, Madeline, Petersen, Benjamin, Pinckard, Jessica, Chandragiri, Siva Sai, Shi, Helen, Reyff, Zeke, Ballard, Cade, Gulej, Rafal, Csik, Boglarka, Ferrier, Jeremy, Balasubramanian, Priya, Yabluchanskiy, Andriy, Cleuren, Audrey, Conley, Shannon, Ungvari, Zoltan, Csiszar, Anna, Tarantini, Stefano
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.11.2024
Schlagworte:
Aging - physiology
Animals
Brain - blood supply
Brain - diagnostic imaging
Cerebrovascular Circulation - physiology
Intravital Microscopy - methods
Male
Mice
Mice, Inbred C57BL
Microbubbles
Ultrasonography - methods
cerebral blood flow
ICONEUS
vascular density
Brain perfusion
rarefaction
ISSN:1559-7016, 1559-7016
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Zusammenfassung:Intra-vital visualization of deep cerebrovascular structures and blood flow in the aging brain has been a difficult challenge in the field of neurovascular research, especially when considering the key role played by the cerebrovasculature in the pathogenesis of both vascular cognitive impairment and dementia (VCID) and Alzheimer's disease (AD). Traditional imaging methods face difficulties with the thicker skull of older brains, making high-resolution imaging and cerebral blood flow (CBF) assessment challenging. However, functional ultrasound (fUS) imaging, an emerging non-invasive technique, provides real-time CBF insights with notable spatial-temporal resolution. This study introduces an enhanced longitudinal fUS method for aging brains. Using elderly (24-month C57BL/6) mice, we detail replacing the skull with a polymethylpentene window for consistent fUS imaging over extended periods. Ultrasound localization mapping (ULM), involving the injection of a microbubble (<<10 μm) suspension allows for recording of high-resolution microvascular vessels and flows. ULM relies on the localization and tracking of single circulating microbubbles in the blood flow. A FIJI-based analysis interprets these high-quality ULM visuals. Testing on older mouse brains, our method successfully unveils intricate vascular specifics even in-depth, showcasing its utility for longitudinal studies that require ongoing evaluations of CBF and vascular aspects in aging-focused research.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1559-7016
1559-7016
DOI:10.1177/0271678X241260526