Transient loading of CD34 + hematopoietic progenitor cells with polystyrene nanoparticles

CD34 hematopoietic progenitor cells (HPCs) offer great opportunities to develop new treatments for numerous malignant and non-malignant diseases. Nanoparticle (NP)-based strategies can further enhance this potential, and therefore a thorough understanding of the loading behavior of HPCs towards NPs...

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Vydáno v:International journal of nanomedicine Ročník 12; s. 459 - 472
Hlavní autoři: Deville, Sarah, Hadiwikarta, Wahyu, Smisdom, Nick, Wathiong, Bart, Ameloot, Marcel, Nelissen, Inge, Hooyberghs, Jef
Médium: Journal Article
Jazyk:angličtina
Vydáno: New Zealand Taylor & Francis Ltd 01.01.2017
Dove Medical Press
Témata:
Acids
Antigens, CD34 - metabolism
Bacterial Proteins - chemistry
Cell Death
Cell Proliferation
Cells, Cultured
dendritic cells
Experiments
Gadolinium oxide
Green Fluorescent Proteins - chemistry
hematopoietic progenitor cells
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
Hemodialysis
Humans
Infant, Newborn
Kinetics
Luminescent Proteins - chemistry
Models, Biological
Nanoparticles
Nanoparticles - chemistry
NMR
Nuclear magnetic resonance
Original Research
Polystyrenes - chemistry
Quantum dots
release
Stem cells
Umbilical cord
uptake
United Kingdom > UK
Belgium
United States > US
Germany
dendritic cells
hematopoietic progenitor cells
nanoparticles
uptake
release
ISSN:1178-2013, 1176-9114, 1178-2013
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Shrnutí:CD34 hematopoietic progenitor cells (HPCs) offer great opportunities to develop new treatments for numerous malignant and non-malignant diseases. Nanoparticle (NP)-based strategies can further enhance this potential, and therefore a thorough understanding of the loading behavior of HPCs towards NPs is essential for a successful application. The present study focusses on the interaction kinetics of 40 nm sized carboxylated polystyrene (PS) NPs with HPCs. Interestingly, a transient association of the NPs with HPCs is observed, reaching a maximum within 1 hour and declining afterwards. This behavior is not seen in dendritic cells (CD34-DCs) differentiated from HPCs, which display a monotonic increase in NP load. We demonstrate that this transient interaction requires an energy-dependent cellular process, suggesting active loading and release of NPs by HPCs. This novel observation offers a unique approach to transiently equip HPCs. A simple theoretical approach modeling the kinetics of NP loading and release is presented, contributing to a framework of describing this phenomenon.
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ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/IJN.S119407