Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL

Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combinat...

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Bibliographic Details
Published in:	Scientific reports Vol. 6; no. 1; p. 29494
Main Authors:	Halliwell, Diane E., Morais, Camilo L. M., Lima, Kássio M. G., Trevisan, Julio, Siggel-King, Michele R. F., Craig, Tim, Ingham, James, Martin, David S., Heys, Kelly A., Kyrgiou, Maria, Mitra, Anita, Paraskevaidis, Evangelos, Theophilou, Georgios, Martin-Hirsch, Pierre L., Cricenti, Antonio, Luce, Marco, Weightman, Peter, Martin, Francis L.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 12.07.2016 Nature Publishing Group
Subjects:	14 631/57/2282 631/67/2321 631/92/56 Adenocarcinoma - diagnostic imaging Adenocarcinoma - pathology Adolescent Adult Algorithms Bioavailability Biomarkers Biomarkers - metabolism Cell Proliferation Cellular biology Cervical cancer Cervical Intraepithelial Neoplasia - diagnostic imaging Cervical Intraepithelial Neoplasia - pathology Cohort Studies Computer Simulation Cytology DNA - chemistry Electrons Female Gynecology Human papillomavirus Humanities and Social Sciences Humans Light microscopy Lipids Lipids - chemistry Microscopy Microscopy - methods Microscopy, Atomic Force Middle Aged Models, Statistical multidisciplinary Nucleic acids Obstetrics Patients Principal Component Analysis Proteins Science Spectroscopy, Fourier Transform Infrared Topography Uterine Cervical Neoplasms - diagnostic imaging Uterine Cervical Neoplasms - pathology Wavelengths Young Adult United Kingdom > UK
ISSN:	2045-2322, 2045-2322
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Summary:	Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep29494