Prolonged maintenance of human hair follicles in vitro in a serum-free medium
We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the prolonged in vitro growth of isolated human hair follicles for at least 9 days. This was achieved after analysis of the contribution of certain c...
Gespeichert in:
| Veröffentlicht in: | British journal of dermatology (1951) Jg. 129; H. 4; S. 372 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
England
01.10.1993
|
| Schlagworte: | |
| ISSN: | 0007-0963 |
| Online-Zugang: | Weitere Angaben |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the prolonged in vitro growth of isolated human hair follicles for at least 9 days. This was achieved after analysis of the contribution of certain components of the original medium and, by a process of elimination, deriving a completely defined medium supplemented only with antibiotics, L-glutamine, insulin and hydrocortisone. We have shown, by [methyl-3H] thymidine autoradiography, that the hair follicles grown in this medium maintain an in vivo pattern of DNA synthesis, and that the gross morphology and histology of these maintained hair follicles remains similar to that of freshly isolated hair follicles. We have also shown that the patterns of keratin synthesis, as determined by [35S] methionine labelling, do not alter with maintenance. |
|---|---|
| AbstractList | We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the prolonged in vitro growth of isolated human hair follicles for at least 9 days. This was achieved after analysis of the contribution of certain components of the original medium and, by a process of elimination, deriving a completely defined medium supplemented only with antibiotics, L-glutamine, insulin and hydrocortisone. We have shown, by [methyl-3H] thymidine autoradiography, that the hair follicles grown in this medium maintain an in vivo pattern of DNA synthesis, and that the gross morphology and histology of these maintained hair follicles remains similar to that of freshly isolated hair follicles. We have also shown that the patterns of keratin synthesis, as determined by [35S] methionine labelling, do not alter with maintenance. We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the prolonged in vitro growth of isolated human hair follicles for at least 9 days. This was achieved after analysis of the contribution of certain components of the original medium and, by a process of elimination, deriving a completely defined medium supplemented only with antibiotics, L-glutamine, insulin and hydrocortisone. We have shown, by [methyl-3H] thymidine autoradiography, that the hair follicles grown in this medium maintain an in vivo pattern of DNA synthesis, and that the gross morphology and histology of these maintained hair follicles remains similar to that of freshly isolated hair follicles. We have also shown that the patterns of keratin synthesis, as determined by [35S] methionine labelling, do not alter with maintenance.We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the prolonged in vitro growth of isolated human hair follicles for at least 9 days. This was achieved after analysis of the contribution of certain components of the original medium and, by a process of elimination, deriving a completely defined medium supplemented only with antibiotics, L-glutamine, insulin and hydrocortisone. We have shown, by [methyl-3H] thymidine autoradiography, that the hair follicles grown in this medium maintain an in vivo pattern of DNA synthesis, and that the gross morphology and histology of these maintained hair follicles remains similar to that of freshly isolated hair follicles. We have also shown that the patterns of keratin synthesis, as determined by [35S] methionine labelling, do not alter with maintenance. |
| Author | Kealey, T Philpott, M P Westgate, G E Gibson, W T |
| Author_xml | – sequence: 1 givenname: G E surname: Westgate fullname: Westgate, G E organization: Unilever Research, Sharnbrook, Bedford, U.K – sequence: 2 givenname: W T surname: Gibson fullname: Gibson, W T – sequence: 3 givenname: T surname: Kealey fullname: Kealey, T – sequence: 4 givenname: M P surname: Philpott fullname: Philpott, M P |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7692925$$D View this record in MEDLINE/PubMed |
| BookMark | eNotT8tOwzAQ9KGolMInIFkcuCWs7dSuj6jiJRXBAc6RY2-oq8QudoLg7wmic5mV5qGdMzILMSAhVwxKNuFmXzIhVwVnQpRMa1EODQgmofyekQUAqAK0FKfkLOc9ABOwgjmZK6m55qsFeX5NsYvhAx3tjQ8DBhMs0tjS3dibQHfGJ9rGrvO2w0x9oF9-SPHvMDRjGvuiTYi0R-fH_pyctKbLeHHkJXm_v3vbPBbbl4enze22sJWQvJBr68ChdYwZvW6FY20jnZVWGwfaqca0ylYKpbZYTSKIKVCZqhFW6pVd8yW5_u89pPg5Yh7q3meLXWcCxjHXSgJXiovJeHk0js30Yn1Ivjfppz7u57_oKWBh |
| CitedBy_id | crossref_primary_10_1111_j_1365_2133_1999_02652_x crossref_primary_10_1371_journal_pone_0271448 crossref_primary_10_1111_j_1365_2133_1997_tb03778_x crossref_primary_10_1016_0923_1811_94_90036_1 crossref_primary_10_1111_1523_1747_ep12398584 crossref_primary_10_1002_lsm_20679 crossref_primary_10_1016_S0733_8635_05_70090_5 crossref_primary_10_1111_1523_1747_ep12382494 crossref_primary_10_1016_S0950_3552_97_80034_X crossref_primary_10_1046_j_0022_202x_2001_01461_x crossref_primary_10_1210_edrv_21_4_0404 crossref_primary_10_1111_j_1365_2133_1994_tb08487_x crossref_primary_10_1111_j_0906_6705_2004_00207_x crossref_primary_10_1016_j_bej_2009_09_008 crossref_primary_10_1111_exd_13669 crossref_primary_10_1111_1523_1747_ep12285625 crossref_primary_10_1371_journal_pone_0174389 crossref_primary_10_1046_j_1523_1747_2000_00063_x crossref_primary_10_1111_exd_12836 crossref_primary_10_1007_BF02505825 crossref_primary_10_1016_S0733_8635_05_70387_9 crossref_primary_10_1038_s41598_017_15038_9 crossref_primary_10_1111_ics_12554 crossref_primary_10_1002_cbf_1751 crossref_primary_10_1002_lsm_23085 crossref_primary_10_1016_S0738_081X_00_00121_8 crossref_primary_10_1111_j_1365_2133_2007_08245_x crossref_primary_10_1016_j_ejcb_2017_03_011 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1111/j.1365-2133.1993.tb03160.x |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine |
| ExternalDocumentID | 7692925 |
| Genre | Journal Article |
| GroupedDBID | --- .3N .55 .GA .GJ .Y3 05W 0R~ 10A 1CY 1OB 1OC 23N 31~ 33P 36B 3SF 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52R 52S 52T 52U 52V 52W 52X 53G 5GY 5HH 5LA 5RE 5VS 5WD 66C 6P2 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A01 A03 AABZA AACZT AAESR AAEVG AAHHS AANHP AAONW AAPGJ AAPXW AARHZ AASGY AAUAY AAVAP AAWDT AAXRX AAZKR ABCQN ABCUV ABDFA ABEJV ABEML ABGNP ABJNI ABNHQ ABOCM ABPTD ABPVW ABQNK ABVGC ABWST ABXGK ABXVV ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACFRR ACGFS ACMXC ACPOU ACPRK ACRPL ACSCC ACUTJ ACXBN ACXQS ACYXJ ACZBC ADBBV ADEOM ADIPN ADIZJ ADKYN ADMGS ADNMO ADOZA ADQBN ADVEK ADVOB ADXAS ADZMN ADZOD AEEZP AEIMD AENEX AEQDE AEUQT AFBPY AFEBI AFFNX AFFQV AFGKR AFPWT AFYAG AFZJQ AGMDO AGQXC AGUTN AHEFC AHMMS AIACR AIURR AIWBW AJAOE AJBDE AJEEA ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB APJGH ASPBG ATGXG ATUGU AVWKF AZBYB AZFZN AZVAB BAFTC BCRHZ BDRZF BHBCM BMXJE BROTX BRXPI BY8 C45 CAG CGR COF CS3 CUY CVF D-6 D-7 D-E D-F DC6 DCZOG DPXWK DR2 DRFUL DRMAN DRSTM DU5 EBS ECM EIF EJD EMOBN ESX EX3 F00 F01 F04 F5P FEDTE FUBAC FZ0 G-S G.N GODZA H.X H13 HF~ HVGLF HZI HZ~ IHE IX1 J0M J5H K48 KBYEO KOP L7B LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MK4 MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM N04 N05 N9A NF~ NPM NU- O66 O9- OAUYM OCZFY OIG OJZSN OPAEJ OVD OWPYF P2P P2W P2X P2Z P4B P4D PALCI Q.N Q11 QB0 R.K RIWAO RJQFR ROL ROX RX1 SUPJJ TEORI TMA UB1 V9Y VVN W8V W99 WBKPD WHWMO WIH WIJ WIK WOHZO WOW WQJ WRC WUP WVDHM WXI X7M XG1 Y6R YFH ZGI ZXP ZZTAW ~IA ~WT 7X8 ACVCV ADMTO AGQPQ AJBYB AJDVS AVNTJ OBFPC |
| ID | FETCH-LOGICAL-c4362-68cd0decd11a98f3d1fb6dc6c9ad09d7baf7c47e69ce4d1f038cd4a4b3c695c82 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 31 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=10_1111_j_1365_2133_1993_tb03160_x&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0007-0963 |
| IngestDate | Sun Sep 28 03:53:21 EDT 2025 Wed Feb 19 02:33:09 EST 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4362-68cd0decd11a98f3d1fb6dc6c9ad09d7baf7c47e69ce4d1f038cd4a4b3c695c82 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 7692925 |
| PQID | 76027723 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_76027723 pubmed_primary_7692925 |
| PublicationCentury | 1900 |
| PublicationDate | October 1993 |
| PublicationDateYYYYMMDD | 1993-10-01 |
| PublicationDate_xml | – month: 10 year: 1993 text: October 1993 |
| PublicationDecade | 1990 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | British journal of dermatology (1951) |
| PublicationTitleAlternate | Br J Dermatol |
| PublicationYear | 1993 |
| SSID | ssj0013050 |
| Score | 1.5637325 |
| Snippet | We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 372 |
| SubjectTerms | Autoradiography Culture Media, Serum-Free Culture Techniques - methods Hair - anatomy & histology Hair - growth & development Hair - metabolism Humans Keratins - biosynthesis Methionine - metabolism Thymidine - analogs & derivatives Thymidine - metabolism |
| Title | Prolonged maintenance of human hair follicles in vitro in a serum-free medium |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/7692925 https://www.proquest.com/docview/76027723 |
| Volume | 129 |
| WOSCitedRecordID | wos10_1111_j_1365_2133_1993_tb03160_x&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEB6qFfHiu1ife_AazGOzmwVBRCxeWnpQ6K3sU3NoUtMH_nx3khS8iAcvIbAEwmR28u3MN_MB3LpIKMa4DRTjYUBVmgbCKR1Im1JnIpWloanFJvholE0mYtyB-00vDNIqNzGxDtSm1Jgjv-MMq41x8jD_DFAzCmurrYDGFnQTD2TQp_nkRw0hTJsGFEzGeT9rR462PJ6a3eUPaNiul2B-NME8y9fvQLP-4QwO_veqh7DfAk3y2HjGEXRscQy7w7aUfgLDceXjXvFuDZlJHBqBkzcsKR2pZfvIh8wr4nBkN_LmSF6Qdb6sSryRxPvtaha4ylqCxfnV7BTeBs-vTy9Bq60QaIqNUizTJjRWmyiSInOJiZxiRjMtpAmF4Uo6rim3TGhL_WKYoMqRpCrRTKQ6i3uwXZSFPQNCXZQ6y11mnfSH7cR_dSeMBw6ZB-40jvpwszHT1PsuFiRkYcvVYroxVB96jaWn82bEhl_xsC1Oz_989AL2agpiTa-7hK7zm9ZewY5eL_NFdV17hL-OxsNvVtfAcQ |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Prolonged+maintenance+of+human+hair+follicles+in+vitro+in+a+serum-free+medium&rft.jtitle=British+journal+of+dermatology+%281951%29&rft.au=Westgate%2C+G+E&rft.au=Gibson%2C+W+T&rft.au=Kealey%2C+T&rft.au=Philpott%2C+M+P&rft.date=1993-10-01&rft.issn=0007-0963&rft.volume=129&rft.issue=4&rft.spage=372&rft_id=info:doi/10.1111%2Fj.1365-2133.1993.tb03160.x&rft_id=info%3Apmid%2F7692925&rft_id=info%3Apmid%2F7692925&rft.externalDocID=7692925 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0007-0963&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0007-0963&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0007-0963&client=summon |