Prolonged maintenance of human hair follicles in vitro in a serum-free medium

We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the prolonged in vitro growth of isolated human hair follicles for at least 9 days. This was achieved after analysis of the contribution of certain c...

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Bibliographic Details
Published in:British journal of dermatology (1951) Vol. 129; no. 4; p. 372
Main Authors: Westgate, G E, Gibson, W T, Kealey, T, Philpott, M P
Format: Journal Article
Language:English
Published: England 01.10.1993
Subjects:
Autoradiography
Culture Media, Serum-Free
Culture Techniques - methods
Hair - anatomy & histology
Hair - growth & development
Hair - metabolism
Humans
Keratins - biosynthesis
Methionine - metabolism
Thymidine - analogs & derivatives
Thymidine - metabolism
ISSN:0007-0963
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Summary:We have previously reported the in vitro growth of human hair follicles for up to 4 days in a partially defined medium containing serum. We now report the prolonged in vitro growth of isolated human hair follicles for at least 9 days. This was achieved after analysis of the contribution of certain components of the original medium and, by a process of elimination, deriving a completely defined medium supplemented only with antibiotics, L-glutamine, insulin and hydrocortisone. We have shown, by [methyl-3H] thymidine autoradiography, that the hair follicles grown in this medium maintain an in vivo pattern of DNA synthesis, and that the gross morphology and histology of these maintained hair follicles remains similar to that of freshly isolated hair follicles. We have also shown that the patterns of keratin synthesis, as determined by [35S] methionine labelling, do not alter with maintenance.
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ISSN:0007-0963
DOI:10.1111/j.1365-2133.1993.tb03160.x