Accuracy estimation of an indirect ELISA for the detection of West Nile Virus antibodies in wild birds using a latent class model

•An indirect ELISA for surveillance of West Nile virus in wild birds was developed.•Serum samples were tested using ELISA and Virus Neutralization Test (VNT) in parallel.•Tests accuracy was evaluated using different Bayesian two latent class models.•A cut-off making ELISA accuracy comparable to VNT...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 248; pp. 202 - 206
Main Authors:	Tamba, Marco, Caminiti, Antonino, Prosperi, Alice, Desprès, Philippe, Lelli, Davide, Galletti, Giorgio, Moreno, Ana, Paternoster, Giulia, Santi, Annalisa, Licata, Elio, Lecollinet, Sylvie, Gelmini, Luca, Rugna, Gianluca, Procopio, Anna, Lavazza, Antonio
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01.10.2017 Elsevier
Subjects:	absorbance Animals Animals, Wild Antibodies, Viral Antibodies, Viral - blood antigens Antigens, Viral - immunology Bayes Theorem Bird Diseases Bird Diseases - diagnosis Bird Diseases - immunology Bird Diseases - virology Birds Birds - virology blood serum blood transfusion Cross Reactions Culicidae Culicidae - virology diagnostic sensitivity diagnostic specificity ELISA Encephalitis Viruses, Japanese - immunology enzootic diseases Enzyme-Linked Immunosorbent Assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Flavivirus Flavivirus - immunology immunoglobulin G Japanese encephalitis virus juveniles Latent class analysis Life Sciences monoclonal antibodies Neutralization Tests Neutralization Tests - methods Neutralization Tests - veterinary Pica pica public health seroprevalence Surveillance Usutu virus West Nile Fever West Nile Fever - diagnosis West Nile Fever - immunology West Nile Fever - veterinary West Nile virus West Nile virus - immunology wild birds Birds Surveillance Latent class analysis Usutu virus West Nile virus ELISA
ISSN:	0166-0934, 1879-0984, 1879-0984
Online Access:	Get full text
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