Combined bromodeoxyuridine immunocapture and terminal-restriction fragment length polymorphism analysis highlights differences in the active soil bacterial metagenome due to Glomus mosseae inoculation or plant species

Summary High numbers of bacteria are associated with arbuscular mycorrhizal (AM) fungi, but their functions and in situ activities are largely unknown and most have never been characterized. The aim of the present study was to study the impact of Glomus mosseae inoculation and plant type on the acti...

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Vydáno v:Environmental microbiology Ročník 7; číslo 12; s. 1952 - 1966
Hlavní autoři: Artursson, Veronica, Finlay, Roger D., Jansson, Janet K.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Oxford, UK Blackwell Science Ltd 01.12.2005
Témata:
Bacillus - chemistry
Bacillus - genetics
Bacillus - growth & development
Bacteria - chemistry
Bacteria - genetics
Bacteria - growth & development
Benomyl - administration & dosage
Bromodeoxyuridine - analysis
Bromodeoxyuridine - immunology
Genome, Bacterial
Glomus mosseae
Immunohistochemistry
Medicago - microbiology
Mycorrhizae - growth & development
Paenibacillus
Plant Roots - microbiology
Polymorphism, Restriction Fragment Length
Rhizobiaceae - genetics
Rhizobiaceae - isolation & purification
Soil Microbiology
Species Specificity
Triticum - microbiology
ISSN:1462-2912, 1462-2920
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Shrnutí:Summary High numbers of bacteria are associated with arbuscular mycorrhizal (AM) fungi, but their functions and in situ activities are largely unknown and most have never been characterized. The aim of the present study was to study the impact of Glomus mosseae inoculation and plant type on the active bacterial communities in soil by using a molecular approach, bromodeoxyuridine (BrdU) immunocapture in combination with terminal‐restriction fragment length polymorphism (T‐RFLP). This approach combined with sequence information from clone libraries, enabled the identification of actively growing populations, within the total bacterial community. Distinct differences in active bacterial community compositions were found according to G. mosseae inoculation, treatment with an antifungal compound (Benomyl) and plant type. The putative identities of the dominant bacterial species that were activated as a result of G. mosseae inoculation were found to be mostly uncultured bacteria and Paenibacillus species. These populations may represent novel bacterial groups that are able to influence the AM relationship and its subsequent effect on plant growth.
Bibliografie:ArticleID:EMI868
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ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1462-2912
1462-2920
DOI:10.1111/j.1462-2920.2005.00868.x