Differential roles of two DDX17 isoforms in the formation of membraneless organelles

The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences called intrinsically disordered regions (IDRs), which are related to the formation of membraneless organelles (MLOs). Here, we reveal that p72...

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Veröffentlicht in:Journal of biochemistry (Tokyo) Jg. 168; H. 1; S. 33
Hauptverfasser: Hirai, Yuya, Domae, Eisuke, Yoshikawa, Yoshihiro, Tomonaga, Keizo
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 01.07.2020
Schlagworte:
Cell Nucleolus - metabolism
Cell Nucleus - metabolism
DEAD-box RNA Helicases - metabolism
Female
HeLa Cells
Humans
Organelles - metabolism
Protein Isoforms
RNA - metabolism
Uterine Cervical Neoplasms - metabolism
Uterine Cervical Neoplasms - pathology
DDX17
nucleolus
stress granules
membraneless organelles
intrinsically disordered regions
ISSN:1756-2651, 1756-2651
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Abstract The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences called intrinsically disordered regions (IDRs), which are related to the formation of membraneless organelles (MLOs). Here, we reveal that p72 is mostly localized to the nucleoplasm, while p82 is localized to the nucleoplasm and nucleoli. Additionally, p82 exhibited slower intranuclear mobility than p72. Furthermore, the enzymatic mutants of both p72 and p82 accumulate into the stress granules. The enzymatic mutant of p82 abolishes nucleolar localization of p82. Our findings suggest the importance of IDRs and enzymatic activity of DEAD-box proteins in the intracellular distribution and formation of MLOs.
AbstractList The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences called intrinsically disordered regions (IDRs), which are related to the formation of membraneless organelles (MLOs). Here, we reveal that p72 is mostly localized to the nucleoplasm, while p82 is localized to the nucleoplasm and nucleoli. Additionally, p82 exhibited slower intranuclear mobility than p72. Furthermore, the enzymatic mutants of both p72 and p82 accumulate into the stress granules. The enzymatic mutant of p82 abolishes nucleolar localization of p82. Our findings suggest the importance of IDRs and enzymatic activity of DEAD-box proteins in the intracellular distribution and formation of MLOs.
The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences called intrinsically disordered regions (IDRs), which are related to the formation of membraneless organelles (MLOs). Here, we reveal that p72 is mostly localized to the nucleoplasm, while p82 is localized to the nucleoplasm and nucleoli. Additionally, p82 exhibited slower intranuclear mobility than p72. Furthermore, the enzymatic mutants of both p72 and p82 accumulate into the stress granules. The enzymatic mutant of p82 abolishes nucleolar localization of p82. Our findings suggest the importance of IDRs and enzymatic activity of DEAD-box proteins in the intracellular distribution and formation of MLOs.The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences called intrinsically disordered regions (IDRs), which are related to the formation of membraneless organelles (MLOs). Here, we reveal that p72 is mostly localized to the nucleoplasm, while p82 is localized to the nucleoplasm and nucleoli. Additionally, p82 exhibited slower intranuclear mobility than p72. Furthermore, the enzymatic mutants of both p72 and p82 accumulate into the stress granules. The enzymatic mutant of p82 abolishes nucleolar localization of p82. Our findings suggest the importance of IDRs and enzymatic activity of DEAD-box proteins in the intracellular distribution and formation of MLOs.
Author Yoshikawa, Yoshihiro
Hirai, Yuya
Tomonaga, Keizo
Domae, Eisuke
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  surname: Hirai
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  givenname: Eisuke
  surname: Domae
  fullname: Domae, Eisuke
  organization: Department of Biochemistry, Osaka Dental University, 8-1, Kuzuha Hanazono-cho, Hirakata, Osaka 573-1121, Japan
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  givenname: Yoshihiro
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  givenname: Keizo
  surname: Tomonaga
  fullname: Tomonaga, Keizo
  organization: Department of Virus Research, Institute for Frontier Life and Medical Sciences (InFRONT), Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
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CitedBy_id crossref_primary_10_1172_JCI148130
crossref_primary_10_1016_j_tibs_2022_10_001
crossref_primary_10_1073_pnas_2022121118
crossref_primary_10_1007_s00401_021_02333_z
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Keywords DDX17
nucleolus
stress granules
membraneless organelles
intrinsically disordered regions
Language English
License The Author(s) 2020. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
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Snippet The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences...
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SubjectTerms Cell Nucleolus - metabolism
Cell Nucleus - metabolism
DEAD-box RNA Helicases - metabolism
Female
HeLa Cells
Humans
Organelles - metabolism
Protein Isoforms
RNA - metabolism
Uterine Cervical Neoplasms - metabolism
Uterine Cervical Neoplasms - pathology
Title Differential roles of two DDX17 isoforms in the formation of membraneless organelles
URI https://www.ncbi.nlm.nih.gov/pubmed/32065632
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