Differential roles of two DDX17 isoforms in the formation of membraneless organelles

The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences called intrinsically disordered regions (IDRs), which are related to the formation of membraneless organelles (MLOs). Here, we reveal that p72...

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Vydáno v:Journal of biochemistry (Tokyo) Ročník 168; číslo 1; s. 33
Hlavní autoři: Hirai, Yuya, Domae, Eisuke, Yoshikawa, Yoshihiro, Tomonaga, Keizo
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 01.07.2020
Témata:
Cell Nucleolus - metabolism
Cell Nucleus - metabolism
DEAD-box RNA Helicases - metabolism
Female
HeLa Cells
Humans
Organelles - metabolism
Protein Isoforms
RNA - metabolism
Uterine Cervical Neoplasms - metabolism
Uterine Cervical Neoplasms - pathology
DDX17
nucleolus
stress granules
membraneless organelles
intrinsically disordered regions
ISSN:1756-2651, 1756-2651
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Popis
Shrnutí:The RNA helicase, DDX17 is a member of the DEAD-box protein family. DDX17 has two isoforms: p72 and p82. The p82 isoform has additional amino acid sequences called intrinsically disordered regions (IDRs), which are related to the formation of membraneless organelles (MLOs). Here, we reveal that p72 is mostly localized to the nucleoplasm, while p82 is localized to the nucleoplasm and nucleoli. Additionally, p82 exhibited slower intranuclear mobility than p72. Furthermore, the enzymatic mutants of both p72 and p82 accumulate into the stress granules. The enzymatic mutant of p82 abolishes nucleolar localization of p82. Our findings suggest the importance of IDRs and enzymatic activity of DEAD-box proteins in the intracellular distribution and formation of MLOs.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1756-2651
1756-2651
DOI:10.1093/jb/mvaa023