New multiple labelling method for improved satellite cell identification in human muscle: application to a cohort of power-lifters and sedentary men

Presently applied methods to identify and quantify human satellite cells (SCs) give discrepant results. We introduce a new immunofluorescence method that simultaneously monitors two SC markers (NCAM and Pax7), the basal lamina and nuclei. Biopsies from power-lifters, power-lifters using anabolic sub...

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Bibliographic Details
Published in:	Histochemistry and cell biology Vol. 132; no. 2; pp. 141 - 157
Main Authors:	Lindström, Mona, Thornell, Lars-Eric
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.08.2009 Springer-Verlag Springer Nature B.V
Subjects:	Anabolic steroids Antibodies, Monoclonal - immunology Biochemistry Biomarkers - analysis Biomedical and Life Sciences Biomedicine Cell Biology Cellbiologi Cellular biology Cohort Studies Developmental Biology Fluorescent Antibody Technique - methods Humans Immunofluorescense Immunohistochemistry Laminin - analysis Laminin - immunology Male Medical Cell Biology MEDICIN MEDICINE medicinsk cellbiologi Morfologi, cellbiologi, patologi Morphology, cell biology, pathology Muscle, Skeletal - chemistry Muscle, Skeletal - cytology Muscular system NCAM Neural Cell Adhesion Molecules - analysis Neural Cell Adhesion Molecules - immunology Original Paper Pax7 PAX7 Transcription Factor - analysis PAX7 Transcription Factor - immunology Satellite Cells, Skeletal Muscle - chemistry Satellite Cells, Skeletal Muscle - cytology Staining and Labeling - methods Stem cells Steroids Trapezius muscle Weight Lifting Anabolic steroids Trapezius muscle Immunofluorescence NCAM Pax7 Stem cells
ISSN:	0948-6143, 1432-119X, 1432-119X
Online Access:	Get full text
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