New multiple labelling method for improved satellite cell identification in human muscle: application to a cohort of power-lifters and sedentary men

Presently applied methods to identify and quantify human satellite cells (SCs) give discrepant results. We introduce a new immunofluorescence method that simultaneously monitors two SC markers (NCAM and Pax7), the basal lamina and nuclei. Biopsies from power-lifters, power-lifters using anabolic sub...

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Bibliographic Details
Published in:Histochemistry and cell biology Vol. 132; no. 2; pp. 141 - 157
Main Authors: Lindström, Mona, Thornell, Lars-Eric
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.08.2009
Springer-Verlag
Springer Nature B.V
Subjects:
Anabolic steroids
Antibodies, Monoclonal - immunology
Biochemistry
Biomarkers - analysis
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cellbiologi
Cellular biology
Cohort Studies
Developmental Biology
Fluorescent Antibody Technique - methods
Humans
Immunofluorescense
Immunohistochemistry
Laminin - analysis
Laminin - immunology
Male
Medical Cell Biology
MEDICIN
MEDICINE
medicinsk cellbiologi
Morfologi, cellbiologi, patologi
Morphology, cell biology, pathology
Muscle, Skeletal - chemistry
Muscle, Skeletal - cytology
Muscular system
NCAM
Neural Cell Adhesion Molecules - analysis
Neural Cell Adhesion Molecules - immunology
Original Paper
Pax7
PAX7 Transcription Factor - analysis
PAX7 Transcription Factor - immunology
Satellite Cells, Skeletal Muscle - chemistry
Satellite Cells, Skeletal Muscle - cytology
Staining and Labeling - methods
Stem cells
Steroids
Trapezius muscle
Weight Lifting
Anabolic steroids
Trapezius muscle
Immunofluorescence
NCAM
Pax7
Stem cells
ISSN:0948-6143, 1432-119X, 1432-119X
Online Access:Get full text
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Summary:Presently applied methods to identify and quantify human satellite cells (SCs) give discrepant results. We introduce a new immunofluorescence method that simultaneously monitors two SC markers (NCAM and Pax7), the basal lamina and nuclei. Biopsies from power-lifters, power-lifters using anabolic substances and untrained subjects were re-examined. Significantly different results from those with staining for NCAM and nuclei were observed. There were three subtypes of SCs; NCAM⁺/Pax7⁺ (94%), NCAM⁺/Pax7⁻ (4%) and NCAM⁻/Pax7⁺ (1%) but large individual variability existed. The proportion of SCs per nuclei within the basal lamina of myofibres (SC/N) was similar for all groups reflecting a balance between the number of SCs and myonuclei to maintain homeostasis. We emphasise that it is important to quantify both SC/N and the number of SCs per fibre. Our multiple marker method is more reliable for SC identification and quantification and can be used to evaluate other markers of muscle progenitor cells.
Bibliography:http://dx.doi.org/10.1007/s00418-009-0606-0
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ISSN:0948-6143
1432-119X
1432-119X
DOI:10.1007/s00418-009-0606-0