New multiple labelling method for improved satellite cell identification in human muscle: application to a cohort of power-lifters and sedentary men

Presently applied methods to identify and quantify human satellite cells (SCs) give discrepant results. We introduce a new immunofluorescence method that simultaneously monitors two SC markers (NCAM and Pax7), the basal lamina and nuclei. Biopsies from power-lifters, power-lifters using anabolic sub...

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Vydané v:	Histochemistry and cell biology Ročník 132; číslo 2; s. 141 - 157
Hlavní autori:	Lindström, Mona, Thornell, Lars-Eric
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.08.2009 Springer-Verlag Springer Nature B.V
Predmet:	Anabolic steroids Antibodies, Monoclonal - immunology Biochemistry Biomarkers - analysis Biomedical and Life Sciences Biomedicine Cell Biology Cellbiologi Cellular biology Cohort Studies Developmental Biology Fluorescent Antibody Technique - methods Humans Immunofluorescense Immunohistochemistry Laminin - analysis Laminin - immunology Male Medical Cell Biology MEDICIN MEDICINE medicinsk cellbiologi Morfologi, cellbiologi, patologi Morphology, cell biology, pathology Muscle, Skeletal - chemistry Muscle, Skeletal - cytology Muscular system NCAM Neural Cell Adhesion Molecules - analysis Neural Cell Adhesion Molecules - immunology Original Paper Pax7 PAX7 Transcription Factor - analysis PAX7 Transcription Factor - immunology Satellite Cells, Skeletal Muscle - chemistry Satellite Cells, Skeletal Muscle - cytology Staining and Labeling - methods Stem cells Steroids Trapezius muscle Weight Lifting Anabolic steroids Trapezius muscle Immunofluorescence NCAM Pax7 Stem cells
ISSN:	0948-6143, 1432-119X, 1432-119X
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Shrnutí:	Presently applied methods to identify and quantify human satellite cells (SCs) give discrepant results. We introduce a new immunofluorescence method that simultaneously monitors two SC markers (NCAM and Pax7), the basal lamina and nuclei. Biopsies from power-lifters, power-lifters using anabolic substances and untrained subjects were re-examined. Significantly different results from those with staining for NCAM and nuclei were observed. There were three subtypes of SCs; NCAM⁺/Pax7⁺ (94%), NCAM⁺/Pax7⁻ (4%) and NCAM⁻/Pax7⁺ (1%) but large individual variability existed. The proportion of SCs per nuclei within the basal lamina of myofibres (SC/N) was similar for all groups reflecting a balance between the number of SCs and myonuclei to maintain homeostasis. We emphasise that it is important to quantify both SC/N and the number of SCs per fibre. Our multiple marker method is more reliable for SC identification and quantification and can be used to evaluate other markers of muscle progenitor cells.
Bibliografia:	http://dx.doi.org/10.1007/s00418-009-0606-0 ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 14 ObjectType-Article-2 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23
ISSN:	0948-6143 1432-119X 1432-119X
DOI:	10.1007/s00418-009-0606-0