Assessment of the GenoType MTBDRsl VER 2.0 compared to the phenotypic drug susceptibility testing and whole genome sequencing for the rapid detection of resistance to fluoroquinolone and second-line injectable drugs among rifampicin-resistant Mycobacterium tuberculosis isolates

Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDR sl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M...

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Bibliographic Details
Published in:Archives of microbiology Vol. 203; no. 7; pp. 3989 - 3996
Main Authors: Kardan-Yamchi, Jalil, Amini, Sirus, Hamzelou, Gholamreza, Rahimi Foroushani, Abbas, Ghodousi, Arash, Cirillo, Daniela Maria, Feizabadi, Mohammad Mehdi
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2021
Springer Nature B.V
Subjects:
Antibiotics
Biochemistry
Biomedical and Life Sciences
Biotechnology
Capreomycin
Cell Biology
Drug resistance
Drugs
Ecology
fluoroquinolones
Gene sequencing
genes
Genomes
genotype
Genotypes
Germany
Kanamycin
Life Sciences
Microbial Ecology
Microbiology
minimum inhibitory concentration
monitoring
mutants
Mutation
Mycobacterium tuberculosis
Original Paper
phenotype
rapid methods
Rifampin
Rrs gene
Strains (organisms)
Tuberculosis
Whole genome sequencing
Germany
Resistance
Mutation
Drug susceptibility testing
Whole genome sequencing
MTBDR
VER 2.0
ISSN:0302-8933, 1432-072X, 1432-072X
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Summary:Molecular techniques have considerable advantages for rapid detection, a reduction of infectiousness, prevention of further resistance development and surveillance of drug-resistant TB. MTBDR sl VER 2.0 was used to detect resistance to second-line anti-tuberculosis drugs on 35 rifampicin-resistant M. tuberculosis (RR-MTB) isolates compared to the minimum inhibitory concentrations (MICs) and whole genome sequencing (WGS). The MTBDR sl VER 2.0 (Hain Life Science, Nehren, Germany) and WGS (San Diego, CA, USA) were performed for tracing mutations in resistant-related genes involved in resistance to fluoroquinolone (FLQ) and second-line injectable drugs. The broth microdilution method using 7H9 Middlebrook media supplemented with OADC was used to determine the MICs. The MTBDR sl VER 2.0 correctly detected 5/6 (83.3%) of FLQ-resistant strains. The MUT1 A1401G (seven strains) and MUT2 G1484T (one strain) mutations in rrs gene were detected in eight AMK/KAN/CAP-resistant strains. Four low-level KAN-resistant strains with the G-10A/C-12T (three strains) and eis C-14T (one strain) mutations in eis gene was diagnosed using MTBDR sl VER 2.0. Five errors were found in detecting resistance to kanamycin and capreomycin compared to the phenotypic drug susceptibility testing and WGS. Failling wild-type bands without improved mutant bands did not indicate a reliable resistance. WGS could efficiently resolve the discrepancies of the results. MTBDR sl showed better performance in detecting XDR strains than pre-XDR.
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ISSN:0302-8933
1432-072X
1432-072X
DOI:10.1007/s00203-021-02387-3