Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo

Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensi...

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Vydáno v:	eLife Ročník 7
Hlavní autoři:	Pfeiffer, Thomas, Poll, Stefanie, Bancelin, Stephane, Angibaud, Julie, Inavalli, VVG Krishna, Keppler, Kevin, Mittag, Manuel, Fuhrmann, Martin, Nägerl, U Valentin
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England eLife Sciences Publications, Ltd 22.06.2018 eLife Sciences Publications Ltd
Témata:	Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Cerebral Cortex - surgery Dendritic Spines - physiology Dendritic Spines - ultrastructure Female Gene Expression Genes, Reporter Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism hippocampus Hippocampus - anatomy & histology Hippocampus - physiology Hippocampus - ultrastructure Image Processing, Computer-Assisted - statistics & numerical data in vivo imaging learning and memory Luminescent Proteins - genetics Luminescent Proteins - metabolism Male Memory - physiology Mice Mice, Transgenic Microscopy, Fluorescence, Multiphoton - instrumentation Microscopy, Fluorescence, Multiphoton - methods Molecular Imaging - instrumentation Molecular Imaging - methods Nerve Net - anatomy & histology Nerve Net - physiology Nerve Net - ultrastructure Neuronal Plasticity - physiology Neuroscience Pyramidal Cells - physiology Pyramidal Cells - ultrastructure spine plasticity super-resolution microscopy synapses Synapses - physiology Synapses - ultrastructure mouse in vivo imaging synapses hippocampus learning and memory neuroscience spine plasticity super-resolution microscopy
ISSN:	2050-084X, 2050-084X
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Shrnutí:	Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a ‘hippocampal window’ based on resection of cortical tissue and a long working distance objective for optical access. We observed a two-fold higher spine density than previous studies and measured a spine turnover of ~40% within 4 days, which depended on spine size. We thus provide direct evidence for a high level of structural rewiring of synaptic circuits and new insights into the structure-dynamics relationship of hippocampal spines. Having established chronic super-resolution microscopy in the hippocampus in vivo, our study enables longitudinal and correlative analyses of nanoscale neuroanatomical structures with genetic, molecular and behavioral experiments.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally. These authors contributed equally to this work. These authors also contributed equally to this work.
ISSN:	2050-084X 2050-084X
DOI:	10.7554/eLife.34700