Ultra-fast RP-HPLC-FD-DAD for quantification of total aflatoxins in maize, rice, wheat, peanut and poultry feed without sample clean up, and population exposure risk assessment in Katsina, Nigeria: an optimization study

This study reports the development and validation of a simple, yet efficient method called the ultra-fast reverse phase high-performance liquid chromatography with fluorescence and photodiode array detector (UF-RP-HPLC-FD-DAD) to extract and quantify the total aflatoxin from grains and poultry feed....

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Bibliographic Details
Published in:Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Vol. 57; no. 7; pp. 541 - 553
Main Authors: Salisu, Baha'uddeen, Anua, Siti Marwanis, Wan Rosli, Wan Ishak, Mazlan, Nurzafirah, Haron, Rosliza
Format: Journal Article
Language:English
Published: England Taylor & Francis 14.07.2022
Taylor & Francis Ltd
Subjects:
Aflatoxin detection
Aflatoxins
Corn
dietary exposure
dietary exposure risk assessment
environmental science
Exposure
fluorescence
fluorescence detector
food contaminant
food security
Grain
Hepatocellular carcinoma
hepatoma
High-performance liquid chromatography
Liquid chromatography
Nigeria
Optimization
Peanuts
photodiode array detector
Photodiodes
Poultry
Poultry feed
Rice
risk
Risk assessment
Wheat
Nigeria
photodiode array detector
fluorescence detector
food contaminant
dietary exposure risk assessment
Aflatoxin detection
food security
ISSN:0360-1234, 1532-4109, 1532-4109
Online Access:Get full text
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Summary:This study reports the development and validation of a simple, yet efficient method called the ultra-fast reverse phase high-performance liquid chromatography with fluorescence and photodiode array detector (UF-RP-HPLC-FD-DAD) to extract and quantify the total aflatoxin from grains and poultry feed. The proposed method is used to determine the total aflatoxin content in 150 samples of maize, rice, wheat, peanut and poultry feed obtained from open markets in a state in Nigeria. The extent of consumer exposure to aflatoxins and the risk of developing hepatocellular carcinoma (HCC) are evaluated. The UF-RP-HPLC-FD-DAD method was found to be satisfactorily accurate, sensitive and reliable as ascertained by its excellent validation outcomes (R 2 > 0.999, LoD < 0.08 ng g −1 , LoQ < 0.2 ng g −1 , recovery = 90-102%). The aflatoxin levels in food grains and poultry feed samples obtained in this study implied a moderate dietary exposure of between 10.67 and 20.77 ng/kg BW/day, in which the risk of developing HCC was estimated to be between 6.27 and 21.40% per 100,000 adults/year. Hence, greater monitoring of marketed food and feed is required, besides the deployment of strict controls and preventive techniques to minimize the population's exposure to a high dietary level of aflatoxins.
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ISSN:0360-1234
1532-4109
1532-4109
DOI:10.1080/03601234.2022.2073151