Specific detection of small ruminant lentiviral nucleic acid sequences located in the proviral long terminal repeat and leader-gag regions using real-time polymerase chain reaction

Real-time polymerase chain reaction (RT-PCR) detection of proviral nucleic acid sequences of small ruminant lentiviruses (SRLV) in blood samples was developed and evaluated. Priming oligonucleotides were designed on the highly conserved 5′ untranslated leader-gag region while those on the long termi...

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Veröffentlicht in:	Journal of virological methods Jg. 147; H. 2; S. 338 - 344
Hauptverfasser:	Brinkhof, J.M.A., van Maanen, C., Wigger, R., Peterson, K., Houwers, D.J.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London Elsevier B.V 01.02.2008 Amsterdam Elsevier New York, NY
Schlagworte:	Animals Biological and medical sciences Diagnosis DNA, Viral - blood Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Genes, gag Goat Diseases - diagnosis Goat Diseases - virology Goats Lentivirus Lentivirus - genetics Lentivirus - isolation & purification Lentivirus Infections - diagnosis Lentivirus Infections - veterinary Lentivirus Infections - virology Lentiviruses Microbiology Proviruses - genetics Real-time PCR Reverse Transcriptase Polymerase Chain Reaction - methods Ruminantia Serology Sheep Sheep Diseases - diagnosis Sheep Diseases - virology Techniques used in virology Terminal Repeat Sequences Virology Sheep Serology Diagnosis Goats Real-time PCR Lentiviruses Microbiology Method Real time Provirus Virology Polymerase chain reaction Vertebrata Mammalia Goat Artiodactyla LTR sequence Veterinary Detection Ungulata Nucleic acid
ISSN:	0166-0934, 1879-0984
Online-Zugang:	Volltext
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