Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes

Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be...

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Bibliographic Details
Published in:Journal of virological methods Vol. 142; no. 1; pp. 218 - 222
Main Authors: Li, Olive T.W., Barr, Ian, Leung, Connie Y.H., Chen, Honglin, Guan, Yi, Peiris, J.S. Malik, Poon, Leo L.M.
Format: Journal Article
Language:English
Published: London Elsevier B.V 01.06.2007
Amsterdam Elsevier
New York, NY
Subjects:
Animals
Base Sequence
Biological and medical sciences
Consensus primers
Consensus Sequence
DNA Primers
Fundamental and applied biological sciences. Psychology
Hemagglutinin Glycoproteins, Influenza Virus - classification
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Humans
Influenza A virus - classification
Influenza A virus - enzymology
Influenza A virus - genetics
Influenza virus
Microbiology
Molecular Sequence Data
Polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase genes
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA Replicase - chemistry
RNA Replicase - genetics
Techniques used in virology
Viral Proteins - chemistry
Viral Proteins - genetics
Virology
Polymerase chain reaction
Polymerase genes
Influenza virus
Consensus primers
Microbiology
Nucleotide sequence
Hemagglutinin
Orthomyxoviridae
Method
Subunit
Influenzavirus
Virology
Infection
Virus
Gene
Viral disease
Influenza
Reverse transcription polymerase chain reaction
Subtype
ISSN:0166-0934, 1879-0984
Online Access:Get full text
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Description
Summary:Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes.
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ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2007.01.015