Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells

Using absorption and fluorescence spectroscopic methods, quantitative cellular uptake of curcumin, an antioxidant and anti-tumor agent from Curcuma longa, was calculated in two types of normal cells: spleen lymphocytes, and NIH3T3 and two tumor cell lines: EL4 and MCF7. Both the uptake and fluoresce...

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Vydáno v:Biochimica et biophysica acta Ročník 1780; číslo 4; s. 673 - 679
Hlavní autoři: Kunwar, A., Barik, A., Mishra, B., Rathinasamy, K., Pandey, R., Priyadarsini, K.I.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Netherlands Elsevier B.V 01.04.2008
Témata:
Animals
Biological Transport
Cell Line, Tumor
Cell Membrane - metabolism
Cell Nucleus - metabolism
Cell Survival - drug effects
Cells, Cultured
Cellular uptake
Curcuma - chemistry
Curcumin
Curcumin - metabolism
Curcumin - pharmacokinetics
Curcumin - pharmacology
Cytotoxicity
Dose-Response Relationship, Drug
Fluorescence
Humans
Lymphocytes - cytology
Lymphocytes - drug effects
Lymphocytes - metabolism
Mice
Microscopy, Confocal
NIH 3T3 Cells
Nuclear localization
Spectrometry, Fluorescence
Time Factors
Tumor cell
Fluorescence
Cytotoxicity
Cellular uptake
Curcumin
Nuclear localization
Tumor cell
ISSN:0304-4165, 0006-3002, 1872-8006
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Shrnutí:Using absorption and fluorescence spectroscopic methods, quantitative cellular uptake of curcumin, an antioxidant and anti-tumor agent from Curcuma longa, was calculated in two types of normal cells: spleen lymphocytes, and NIH3T3 and two tumor cell lines: EL4 and MCF7. Both the uptake and fluorescence intensity of curcumin were significantly higher in tumor cells compared to the normal cells. A linear dependency on the uptake was observed with treatment concentration of curcumin. Using laser confocal microscopy, intracellular localization of curcumin was monitored and the results indicated that curcumin is located both in the cell membrane and the nucleus. Sub-cellular fractionation of curcumin-loaded MCF7 cells supported the differential distribution of curcumin in membrane, cytoplasm and nuclear compartments of cell with maximum localization in the membrane. Cytotoxicity studies in different cell lines indicated that the toxicity of curcumin increased with increasing uptake.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/j.bbagen.2007.11.016