An evolution-based model for designing chorismate mutase enzymes

The rational design of enzymes is an important goal for both fundamental and practical reasons. Here, we describe a process to learn the constraints for specifying proteins purely from evolutionary sequence data, design and build libraries of synthetic genes, and test them for activity in vivo using...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) Jg. 369; H. 6502; S. 440
Hauptverfasser:	Russ, William P, Figliuzzi, Matteo, Stocker, Christian, Barrat-Charlaix, Pierre, Socolich, Michael, Kast, Peter, Hilvert, Donald, Monasson, Remi, Cocco, Simona, Weigt, Martin, Ranganathan, Rama
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 24.07.2020
Schlagworte:	Amino Acid Sequence Chorismate Mutase - chemistry Chorismate Mutase - genetics Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Evolution, Molecular Models, Genetic Models, Statistical
ISSN:	1095-9203, 1095-9203
Online-Zugang:	Weitere Angaben
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