Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR

Crude cell lysates are increasingly used as input for direct analysis by reverse transcription quantitative PCR (RT-qPCR), particularly for high-throughput applications. We previously demonstrated that a simple buffer containing a non-ionic detergent can serve as an inexpensive alternative to commer...

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Bibliographic Details
Published in:	Analytical biochemistry Vol. 513; pp. 21 - 27
Main Authors:	Wang, Xiao, Teferedegne, Belete, Shatzkes, Kenneth, Tu, Wei, Murata, Haruhiko
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 15.11.2016
Subjects:	A549 Cells Animals Cell lysate Cercopithecus aethiops cost effectiveness detergents Dogs Enzyme Inhibitors - chemistry HeLa Cells Humans Madin Darby Canine Kidney Cells quantitative polymerase chain reaction reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription quantitative PCR ribonucleases Ribonucleases - antagonists & inhibitors Ribonucleases - chemistry RNA RNase RNase inhibitor transcription (genetics) Vero Cells virology Virus Virus Cell lysate Reverse transcription quantitative PCR RNA RNase inhibitor RNase
ISSN:	0003-2697, 1096-0309
Online Access:	Get full text
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