Detachment and sonoporation of adherent HeLa-cells by shock wave-induced cavitation

The interaction of lithotripter-generated shock waves with adherent cells is investigated using high-speed optical techniques. We show that shock waves permeabilize adherent cells in vitro through the action of cavitation bubbles. The bubbles are formed in the trailing tensile pulse of a lithotripte...

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Bibliographic Details
Published in:Biochimica et biophysica acta Vol. 1624; no. 1; pp. 131 - 138
Main Authors: Ohl, Claus-Dieter, Wolfrum, Bernhard
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 05.12.2003
Subjects:
Cavitation
Cell Adhesion
Cell detachment
Cell Survival
Drug delivery
HeLa Cells
High-Energy Shock Waves
Humans
Shock wave
Sonoporation
Ultrasonics
Cell detachment
Drug delivery
Cavitation
Sonoporation
Shock wave
ISSN:0304-4165, 0006-3002, 1872-8006
Online Access:Get full text
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Summary:The interaction of lithotripter-generated shock waves with adherent cells is investigated using high-speed optical techniques. We show that shock waves permeabilize adherent cells in vitro through the action of cavitation bubbles. The bubbles are formed in the trailing tensile pulse of a lithotripter-generated shock wave where the pressure drops below the vapor pressure. Upon collapse of cavitation bubbles, a strong flow field is generated which accounts for two effects: first, detachment of cells from the substrate; and second, the temporary opening of cell membranes followed by molecular uptake, a process called sonoporation. Comparison of observed cell detachment with results from a theoretical model considering peeling cell detachment by a wall jet-induced shear stress shows reasonable agreement.
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/j.bbagen.2003.10.005