Altered decidual and placental catabolism of vitamin D may contribute to the aetiology of spontaneous miscarriage

Vitamin D catabolizing enzymes, along with vitamin D receptor (VDR) and vitamin D binding protein (DBP) are expressed in the decidua and placenta during pregnancy and capable of synthesizing active vitamin D. Vitamin D plays roles in immunoregulation and trophoblast invasion, key features of a succe...

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Published in:Placenta (Eastbourne) Vol. 92; pp. 1 - 8
Main Authors: Hou, H., Zhang, J.Y., Chen, D., Deng, F., Morse, A.N., Qiu, X., He, P., Lash, G.E.
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01.03.2020
Subjects:
25-Hydroxyvitamin D3 1-alpha-Hydroxylase - metabolism
Abortion, Spontaneous - blood
Abortion, Spontaneous - enzymology
Abortion, Spontaneous - etiology
Case-Control Studies
Cholestanetriol 26-Monooxygenase - metabolism
CYP24A1
CYP27B1
Cytochrome P-450 Enzyme System - metabolism
Cytochrome P450 Family 2 - metabolism
Decidua - enzymology
Female
Humans
Maternal-fetal interface
Pregnancy
Receptors, Calcitriol - metabolism
Spontaneous miscarriage
Vitamin D
Vitamin D - metabolism
Vitamin D-Binding Protein - metabolism
Vitamin D3 24-Hydroxylase - metabolism
CYP27B1
Maternal-fetal interface
CYP24A1
Vitamin D
Spontaneous miscarriage
ISSN:0143-4004, 1532-3102, 1532-3102
Online Access:Get full text
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Summary:Vitamin D catabolizing enzymes, along with vitamin D receptor (VDR) and vitamin D binding protein (DBP) are expressed in the decidua and placenta during pregnancy and capable of synthesizing active vitamin D. Vitamin D plays roles in immunoregulation and trophoblast invasion, key features of a successful pregnancy. Epidemiological data suggests that vitamin D deficiency is associated with both spontaneous and recurrent miscarriage but few studies have investigated the expression of the key vitamin D catabolizing enzymes in miscarriage. Placenta and decidua were collected after termination of apparently normal pregnancies (controls, n = 22) or spontaneous miscarriage (n = 20). Immunohistochemical staining, Western Blot and qRT-PCR were performed for CYP27B1, CYP24A1, CYP2R1, VDR and DBP (not qRT-PCR). HTR-8/SVneo cells were cultured in CoCL2 (hypoxic mimetic) or LPS (bacterial infection mimetic) for 24 h, RNA extracted and qRT-PCR performed for CYP27B1, CYP24A1, CYP2R1 and VDR. In spontaneous miscarriage, placental and decidual expression of CYP27B1 was reduced, while expression of CYP24A1, VDR and DBP was increased. When a trophoblast cell line was treated with CoCL2 expression of CYP27B1 was increased and CYP24A1 was reduced, while LPS induced expression of VDR. This is the first report of altered utero-placental vitamin D catabolism in spontaneous miscarriage. It is becoming accepted that women who are undergoing assisted reproductive technologies should ensure they have sufficient vitamin D levels prior to pregnancy, these data support that all women should ensure they are vitamin D replete before planning to get pregnant. •Placental/decidual levels of vitamin D are decreased in spontaneous miscarriage.•CYP enzyme expression changes may be linked to hyperoxia.•VDR expression changes may be linked to infection.
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ISSN:0143-4004
1532-3102
1532-3102
DOI:10.1016/j.placenta.2020.01.013