Osteogenic potential of gingival stromal progenitor cells cultured in platelet rich fibrin is predicted by core-binding factor subunit-α1/Sox9 expression ratio (in vitro)

Background: Alveolar bone defect regeneration has long been problematic in the field of dentistry. Gingival stromal progenitor cells (GSPCs) offer a promising solution for alveolar bone regeneration. In order to optimally differentiate and proliferate progenitor cells, growth factors (GFs) are requi...

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Vydané v:F1000 research Ročník 7; s. 1134
Hlavní autori: Nugraha, Alexander Patera, Narmada, Ida Bagus, Ernawati, Diah Savitri, Dinaryanti, Aristika, Hendrianto, Eryk, Ihsan, Igo Syaiful, Riawan, Wibi, Rantam, Fedik Abdul
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England Faculty of 1000 Ltd 2018
F1000 Research Limited
F1000 Research Ltd
Predmet:
Alveolar bone
Bone (long)
Bone growth
Bones
CBF protein
Copyright
Defects
Dental caries
Dentistry
Fibrin
Growth factors
Immunology
Laboratories
Medicine
Mineralization
Monoclonal antibodies
Osteogenesis
Platelets
Progenitor cells
R&D
Random number sampling
Regeneration
Research & development
Sox9 protein
Statistical sampling
Stem cells
Tissue engineering
Indonesia
Core-Binding Factor Subunit-α
Sox9
Platelet Rich Fibrin
Osteogenic Differentiation
Gingival Stromal Progenitor Cells
ISSN:2046-1402, 2046-1402
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Shrnutí:Background: Alveolar bone defect regeneration has long been problematic in the field of dentistry. Gingival stromal progenitor cells (GSPCs) offer a promising solution for alveolar bone regeneration. In order to optimally differentiate and proliferate progenitor cells, growth factors (GFs) are required. Platelet rich fibrin (PRF) has many GFs and can be easily manufactured. Core-binding factor subunit-α1 (CBF-α1) constitutes a well-known osteogenic differentiation transcription factor in SPCs. Sox9, as a chondrogenic transcription factor, interacts and inhibits CBF-α1, but its precise role in direct in vitro osteogenesis remains unknown. GSPCs cultured in vitro in PRF to optimally stimulate osteogenic differentiation has been largely overlooked. The aim of this study was to analyze GSPCs cultured in PRF osteogenic differentiation predicted by CBF-α1/Sox9. Methods : This study used a true experimental with post-test only control group design and random sampling. GPSCs isolated from the lower gingiva of four healthy, 250-gram, 1-month old, male Wistar rats ( Rattus Novergicus ) were cultured for two weeks, passaged every 4-5 days. GSPCs in passage 3-5 were cultured in five M24 plates (N=108; n=6/group) for Day 7, Day 14, and Day 21 in three different mediums (control negative group: αModified Eagle Medium; control positive group: High Glucose-Dulbecco’s Modified Eagle Medium (DMEM-HG) + osteogenic medium; Treatment group: DMEM-HG + osteogenic medium + PRF). CBF-α1 and Sox9 were examined with ICC monoclonal antibody. A one-way ANOVA continued with Tukey HSD test (p<0.05) based on Kolmogorov–Smirnov and Levene's tests (p>0.05) was performed. Results: The treatment group showed the highest CBF-α1/Sox9 ratio (16.00±3.000/14.33±2.517) on Day 7, while the lowest CBF-α1/Sox9 ratio (3.33±1.528/3.67±1.155) occurred in the control negative group on Day 21, with significant difference between the groups (p<0.05). Conclusion: GSPCs cultured in PRF had potential osteogenic differentiation ability predicted by the CBF-α1/sox9 ratio.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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No competing interests were disclosed.
ISSN:2046-1402
2046-1402
DOI:10.12688/f1000research.15423.1