Genetic diversity of Plasmodium falciparum parasites from Kenya is not affected by antifolate drug selection

The genotypes of merozoite surface protein-1, merozoite surface protein-2 and glutamine rich protein are frequently used to distinguish recrudescence from reinfection when parasitaemia reappears after antimalarial drug treatment. However, none of the previous reports has clearly assessed the change...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	International journal for parasitology Ročník 32; číslo 12; s. 1469 - 1476
Hlavní autoři:	Nzila, A.M, Mberu, E.K, Nduati, E, Ross, A, Watkins, W.M, Sibley, C.H
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford Elsevier Ltd 01.11.2002 Elsevier Science
Témata:	Age Distribution Alleles Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antifolates Antigens, Protozoan - genetics Antimalarials - pharmacology Antimalarials - therapeutic use Antiparasitic agents Biological and medical sciences Body Temperature Child, Preschool Drug Resistance - genetics Fever - complications Fever - parasitology Folic Acid Antagonists - pharmacology Folic Acid Antagonists - therapeutic use Fundamental and applied biological sciences. Psychology Genes, Protozoan - genetics Genetic Variation - drug effects Genetic Variation - genetics Genetics of eukaryotes. Biological and molecular evolution Humans Infant Kenya Malaria - drug therapy Malaria - parasitology Malaria genetic diversity Medical sciences Merozoite Surface Protein 1 - genetics Parasitemia - drug therapy Parasitemia - parasitology Pharmacology. Drug treatments Plasmodium falciparum Plasmodium falciparum - drug effects Plasmodium falciparum - genetics Plasmodium falciparum - growth & development Plasmodium falciparum - isolation & purification Polymorphism, Genetic - drug effects Polymorphism, Genetic - genetics Population genetics, reproduction patterns Protozoa Protozoan Proteins - genetics Kenya Africa Antifolates Plasmodium falciparum Malaria genetic diversity Protozoa Host parasite relation Antimalarial Apicomplexa Protozoal disease Malaria Parasite Artificial selection Population structure Genetic diversity Parasitosis Population genetics Infection Antifolate Antiprotozoal agent Chemotherapy Parasiticid Polymorphism
ISSN:	0020-7519, 1879-0135
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	The genotypes of merozoite surface protein-1, merozoite surface protein-2 and glutamine rich protein are frequently used to distinguish recrudescence from reinfection when parasitaemia reappears after antimalarial drug treatment. However, none of the previous reports has clearly assessed the change of genetic diversity following drug treatment. In the present study, we have assessed the impact of pyrimethamine/sulfadoxine and chlorproguanil/dapsone on the genetic diversity of isolates and the multiplicity of infection in patient isolates from Kilifi, Kenya. We have analysed the length polymorphism of merozoite surface protein-1, merozoite surface protein-2 and glutamine rich protein and the data clearly show that treatment with pyrimethamine/sulfadoxine and chlorproguanil/dapsone did not change the multiplicity of infection found in patients, in contrast to the selection that these drugs exert on the genes encoded by the target enzymes. In addition, we report that children of less than 2 years tend to have fewer numbers of clones per isolate when compared with older children. Overall, this study shows that the selection for genes that confer drug resistance is not a factor in reducing the genetic diversity of parasite clones in a patient.
Bibliografie:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0020-7519 1879-0135
DOI:	10.1016/S0020-7519(02)00164-9