Genetic diversity of Plasmodium falciparum parasites from Kenya is not affected by antifolate drug selection

The genotypes of merozoite surface protein-1, merozoite surface protein-2 and glutamine rich protein are frequently used to distinguish recrudescence from reinfection when parasitaemia reappears after antimalarial drug treatment. However, none of the previous reports has clearly assessed the change...

Full description

Saved in:
Bibliographic Details
Published in:International journal for parasitology Vol. 32; no. 12; pp. 1469 - 1476
Main Authors: Nzila, A.M, Mberu, E.K, Nduati, E, Ross, A, Watkins, W.M, Sibley, C.H
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 01.11.2002
Elsevier Science
Subjects:
Age Distribution
Alleles
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antifolates
Antigens, Protozoan - genetics
Antimalarials - pharmacology
Antimalarials - therapeutic use
Antiparasitic agents
Biological and medical sciences
Body Temperature
Child, Preschool
Drug Resistance - genetics
Fever - complications
Fever - parasitology
Folic Acid Antagonists - pharmacology
Folic Acid Antagonists - therapeutic use
Fundamental and applied biological sciences. Psychology
Genes, Protozoan - genetics
Genetic Variation - drug effects
Genetic Variation - genetics
Genetics of eukaryotes. Biological and molecular evolution
Humans
Infant
Kenya
Malaria - drug therapy
Malaria - parasitology
Malaria genetic diversity
Medical sciences
Merozoite Surface Protein 1 - genetics
Parasitemia - drug therapy
Parasitemia - parasitology
Pharmacology. Drug treatments
Plasmodium falciparum
Plasmodium falciparum - drug effects
Plasmodium falciparum - genetics
Plasmodium falciparum - growth & development
Plasmodium falciparum - isolation & purification
Polymorphism, Genetic - drug effects
Polymorphism, Genetic - genetics
Population genetics, reproduction patterns
Protozoa
Protozoan Proteins - genetics
Kenya
Africa
Antifolates
Plasmodium falciparum
Malaria genetic diversity
Protozoa
Host parasite relation
Antimalarial
Apicomplexa
Protozoal disease
Malaria
Parasite
Artificial selection
Population structure
Genetic diversity
Parasitosis
Population genetics
Infection
Antifolate
Antiprotozoal agent
Chemotherapy
Parasiticid
Polymorphism
ISSN:0020-7519, 1879-0135
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The genotypes of merozoite surface protein-1, merozoite surface protein-2 and glutamine rich protein are frequently used to distinguish recrudescence from reinfection when parasitaemia reappears after antimalarial drug treatment. However, none of the previous reports has clearly assessed the change of genetic diversity following drug treatment. In the present study, we have assessed the impact of pyrimethamine/sulfadoxine and chlorproguanil/dapsone on the genetic diversity of isolates and the multiplicity of infection in patient isolates from Kilifi, Kenya. We have analysed the length polymorphism of merozoite surface protein-1, merozoite surface protein-2 and glutamine rich protein and the data clearly show that treatment with pyrimethamine/sulfadoxine and chlorproguanil/dapsone did not change the multiplicity of infection found in patients, in contrast to the selection that these drugs exert on the genes encoded by the target enzymes. In addition, we report that children of less than 2 years tend to have fewer numbers of clones per isolate when compared with older children. Overall, this study shows that the selection for genes that confer drug resistance is not a factor in reducing the genetic diversity of parasite clones in a patient.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(02)00164-9