Cutaneous MMPs are differently modulated by environmental stressors in old and young mice

Skin is frequently exposed to pro-oxidative insults such as UV light, ozone (O 3) and cigarette smoke (CS), which are able to deplete antioxidants and induce oxidation products affecting skin pathophysiology. Skin turnover and regeneration are largely dependent on extracellular matrix metabolism, wh...

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Vydáno v:	Toxicology letters Ročník 173; číslo 2; s. 73 - 79
Hlavní autoři:	Fortino, Vittoria, Maioli, Emanuela, Torricelli, Claudia, Davis, Paul, Valacchi, Giuseppe
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Shannon Elsevier Ireland Ltd 10.09.2007 Amsterdam Elsevier Science
Témata:	Aging - metabolism Animals Biological and medical sciences Cigarette smoke Enzyme Induction Extracellular Matrix - metabolism Female Matrix Metalloproteinase 12 - biosynthesis Matrix Metalloproteinase 2 - biosynthesis Matrix Metalloproteinase 9 - biosynthesis Matrix Metalloproteinases - biosynthesis Medical sciences Mice Mice, Hairless MMPs Nicotiana Oxidants, Photochemical - pharmacology Oxidative Stress Ozone Ozone - pharmacology Skin Skin - drug effects Skin - enzymology Skin - radiation effects Smoke - adverse effects TIMPs Tissue Inhibitor of Metalloproteinase-1 - metabolism Tissue Inhibitor of Metalloproteinase-2 - metabolism Tobacco, tobacco smoking Toxicology Ultraviolet Rays UV Ozone TIMPs Skin Cigarette smoke MMPs Enzyme Rodentia Tobacco smoking Metalloendopeptidases Tissue inhibitor metalloproteinase Stress Cigarette Peptidases Vertebrata Tobacco Mammalia Mouse Animal Hydrolases Environment Fumes
ISSN:	0378-4274, 1879-3169
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Popis
Shrnutí:	Skin is frequently exposed to pro-oxidative insults such as UV light, ozone (O 3) and cigarette smoke (CS), which are able to deplete antioxidants and induce oxidation products affecting skin pathophysiology. Skin turnover and regeneration are largely dependent on extracellular matrix metabolism, which is under the control of matrix metalloproteinases, MMPs. The present study evaluated cutaneous MMPs activity upon environmental pollutants exposure and analyzed the response of old and young animals. For this purpose, SKH-1 hairless mice (8 weeks and 18 months old) were exposed for 6 h/day to 0.25 ppm of O 3 or to UV radiation (0.3 MED) or to CS for 4 days. Gelatin zymography revealed an increase of MMP-2 in both young and old animals, after exposure to pollutants, while MMP-9, undetectable in unexposed subjects, was strongly induced only in old mice. Casein zymography and Western blot analysis showed an increase of MMP-12 in the aged group after environmental stressors exposure. TIMP-1 and -2 expression levels did not change. The current study demonstrates the ability of certain environmental pollutants to affect the ECM turnover through modulation of specific MMPs, and confirms the higher susceptibility of old subjects to exogenous pro-oxidant insults.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0378-4274 1879-3169
DOI:	10.1016/j.toxlet.2007.06.004