Synthetic Biology Enables Programmable Cell‐Based Biosensors

Cell‐based biosensors offer cheap, portable and simple methods of detecting molecules of interest but have yet to be truly adopted commercially. Issues with their performance and specificity initially slowed the development of cell‐based biosensors. With the development of rational approaches to tun...

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Vydáno v:Chemphyschem Ročník 21; číslo 2; s. 132 - 144
Hlavní autoři: Hicks, Maggie, Bachmann, Till T., Wang, Baojun
Médium: Journal Article
Jazyk:angličtina
Vydáno: Germany Wiley Subscription Services, Inc 16.01.2020
John Wiley and Sons Inc
Témata:
Bacteria - cytology
Biology
Biosensing Techniques - instrumentation
Biosensors
Cell Engineering
cell-based biosensor
genetic circuits
Humans
rational approaches
response curve
Review
Reviews
Stability
Synthetic Biology
synthetic biology
response curve
genetic circuits
rational approaches
cell-based biosensor
ISSN:1439-4235, 1439-7641, 1439-7641
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Shrnutí:Cell‐based biosensors offer cheap, portable and simple methods of detecting molecules of interest but have yet to be truly adopted commercially. Issues with their performance and specificity initially slowed the development of cell‐based biosensors. With the development of rational approaches to tune response curves, the performance of biosensors has rapidly improved and there are now many biosensors capable of sensing with the required performance. This has stimulated an increased interest in biosensors and their commercial potential. However the reliability, long term stability and biosecurity of these sensors are still barriers to commercial application and public acceptance. Research into overcoming these issues remains active. Here we present the state‐of‐the‐art tools offered by synthetic biology to allow construction of cell‐based biosensors with customisable performance to meet the real world requirements in terms of sensitivity and dynamic range and discuss the research progress to overcome the challenges in terms of the sensor stability and biosecurity fears. Sensor in a cell: This review presents the state‐of‐the‐art tools offered by synthetic biology to allow construction of cell‐based biosensors with customisable performance to meet the real‐world requirements in terms of sensitivity, selectivity, dynamic range and biosecurity.
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ISSN:1439-4235
1439-7641
1439-7641
DOI:10.1002/cphc.201900739