Production of Trichophyton rubrum microspores in large quantities and its application to evaluate amorolfine/azole compound interactions in vitro

Summary Trichophyton rubrum is the most frequently isolated dermatophyte species in European countries. The lack or poor sporulation of T. rubrum has always been a major complication and a limiting factor when performing antifungal susceptibility testing. Therefore, we describe an in vitro method ai...

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Bibliographic Details
Published in:Mycoses Vol. 60; no. 9; pp. 581 - 586
Main Authors: Laurent, Alexis, Monod, Michel
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01.09.2017
Subjects:
Amorolfine
Antifungal Agents - pharmacology
azole compounds
Azoles - pharmacology
Carbon Dioxide
Culture Media
Data processing
Europe
Humans
isobolograms
Microbial Sensitivity Tests
Microbiological Techniques
Morpholines - pharmacology
Spores, Fungal - drug effects
Spores, Fungal - isolation & purification
Sporulation
synergism
Trichophyton - drug effects
Trichophyton - growth & development
Trichophyton - physiology
Trichophyton rubrum
synergism
Trichophyton rubrum
azole compounds
amorolfine
isobolograms
sporulation
ISSN:0933-7407, 1439-0507, 1439-0507
Online Access:Get full text
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Summary:Summary Trichophyton rubrum is the most frequently isolated dermatophyte species in European countries. The lack or poor sporulation of T. rubrum has always been a major complication and a limiting factor when performing antifungal susceptibility testing. Therefore, we describe an in vitro method aiming to enhance sporulation of various T. rubrum isolates in order to perform antifungigrams. A combination of high CO2 tensions and incubation on PDA growth medium revealed to be optimal for sporulation of all tested T. rubrum isolates. This method was further used to examine in vitro the combined effects of amorolfine and azole derivatives against fungal growth using adapted checkerboard microdilution assays and an isobolographic approach of the data, adapted disc diffusion and Etest assays. Non‐antagonistic and synergistic effects were observed in these settings with amorolfine combined to each of the tested azole compounds. The optimised culture method appeared to be suitable for T. rubrum isolates for which antifungigrams were especially difficult to obtain because of the lack of sporulation.
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ISSN:0933-7407
1439-0507
1439-0507
DOI:10.1111/myc.12632