Synergistic interactions of Pseudomonas aeruginosa and Staphylococcus aureus in an in vitro wound model

In individuals with polymicrobial infections, microbes often display synergistic interactions that can enhance their colonization, virulence, or persistence. One of the most prevalent types of polymicrobial infection occurs in chronic wounds, where Pseudomonas aeruginosa and Staphylococcus aureus ar...

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Veröffentlicht in:	Infection and immunity Jg. 82; H. 11; S. 4718
Hauptverfasser:	DeLeon, Stephanie, Clinton, Allie, Fowler, Haley, Everett, Jake, Horswill, Alexander R, Rumbaugh, Kendra P
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.11.2014
Schlagworte:	Anti-Bacterial Agents - pharmacology Bacteriological Techniques - methods Coculture Techniques - methods Drug Resistance, Bacterial - physiology Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - physiology Staphylococcus aureus - drug effects Staphylococcus aureus - physiology
ISSN:	1098-5522, 1098-5522
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Zusammenfassung:	In individuals with polymicrobial infections, microbes often display synergistic interactions that can enhance their colonization, virulence, or persistence. One of the most prevalent types of polymicrobial infection occurs in chronic wounds, where Pseudomonas aeruginosa and Staphylococcus aureus are the two most common causes. Although they are the most commonly associated microbial species in wound infections, very little is known about their interspecies relationship. Evidence suggests that P. aeruginosa-S. aureus coinfections are more virulent than monoculture infection with either species; however, difficulties in growing these two pathogens together in vitro have hampered attempts to uncover the mechanisms involved. Here we describe a simple and clinically relevant in vitro wound model that supported concomitant growth of P. aeruginosa and S. aureus. We observed that the ability of P. aeruginosa and S. aureus to survive antibiotic treatment increased when they were grown together in planktonic cocultures and that antibiotic tolerance was further enhanced when they were grown together in the wound model. We attributed this enhanced tolerance to both the "host-derived" and "bacterium-derived" matrix components. Taken together, our data indicate that P. aeruginosa and S. aureus may benefit each other by coinfecting wounds and that the host-derived matrix may serve as important a role as the bacterium-derived matrix in protecting bacteria from some antibiotics.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1098-5522 1098-5522
DOI:	10.1128/IAI.02198-14