Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Plaque assays remain one of the most accurate methods for the direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture. Here we demonstrate how to perform a basic plaque assay, and how diff...

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Bibliographic Details

Published in:	Journal of visualized experiments no. 93; p. e52065
Main Authors:	Baer, Alan, Kehn-Hall, Kylene
Format:	Journal Article
Language:	English
Published:	United States MyJove Corporation 04.11.2014
Subjects:	agarose Animals carboxymethylcellulose Carboxymethylcellulose Sodium cell culture Cellulose culture media heat Humans liquids polymers Viral Plaque Assay - methods Virology viruses Viruses - isolation & purification
ISSN:	1940-087X, 1940-087X
Online Access:	Get full text
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