Preparation and in vitro evaluation of folate-receptor-targeted SPION-polymer micelle hybrids for MRI contrast enhancement in cancer imaging

Polymer-SPION hybrids were investigated for receptor-mediated localization in tumour tissue. Superparamagnetic iron oxide nanoparticles (SPIONs) prepared by high-temperature decomposition of iron acetylacetonate were monodisperse (9.27 ± 3.37 nm), with high saturation magnetization of 76.8 emu g(-1)...

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Published in:Nanotechnology Vol. 24; no. 1; p. 015603
Main Authors: Mahajan, Shveta, Koul, Veena, Choudhary, Veena, Shishodia, Gauri, Bharti, Alok C
Format: Journal Article
Language:English
Published: England 11.01.2013
Subjects:
Cell Death - drug effects
Contrast Media
Dextrans - chemical synthesis
Dextrans - chemistry
Dextrans - toxicity
Dextrans - ultrastructure
Diagnostic Imaging - methods
Endocytosis - drug effects
Folate Receptor 1 - metabolism
HeLa Cells
Humans
Intracellular Space - drug effects
Intracellular Space - metabolism
Magnetic Resonance Imaging
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - toxicity
Magnetite Nanoparticles - ultrastructure
Micelles
Microscopy, Confocal
Microscopy, Electron, Transmission
Neoplasms - diagnosis
Neoplasms - pathology
Polymerization
Polymers - chemical synthesis
Polymers - chemistry
Pyrenes - chemistry
Spectrophotometry, Ultraviolet
X-Ray Diffraction
ISSN:1361-6528, 1361-6528
Online Access:Get more information
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Summary:Polymer-SPION hybrids were investigated for receptor-mediated localization in tumour tissue. Superparamagnetic iron oxide nanoparticles (SPIONs) prepared by high-temperature decomposition of iron acetylacetonate were monodisperse (9.27 ± 3.37 nm), with high saturation magnetization of 76.8 emu g(-1). Amphiphilic copolymers prepared from methyl methacrylate and PEG methacrylate by atom transfer radical polymerization were conjugated with folic acid (for folate-receptor specificity). The folate-conjugated polymer had a low critical micellar concentration (0.4 mg l(-1)), indicating stability of the micellar formulation. SPION-polymeric micelle clusters were prepared by desolvation of the SPION dispersion/polymer solution in water. Magnetic resonance imaging of the formulation revealed very good contrast enhancement, with transverse (T(2)) relaxivity of 260.4 mM(-1) s(-1). The biological evaluation of the SPION micelles included cellular viability assay (MTT) and uptake in HeLa cells. These studies demonstrated the potential use of these nanoplatforms for imaging and targeting.
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ISSN:1361-6528
1361-6528
DOI:10.1088/0957-4484/24/1/015603