Association of 'Candidatus Liberibacter solanacearum' with a vegetative disorder of celery in Spain and development of a real-time PCR method for its detection

A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with 'Candidatus Liberibacter solanacearum' and not with phytoplasmas. Sampl...

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Veröffentlicht in:Phytopathology Jg. 104; H. 8; S. 804
Hauptverfasser: Teresani, Gabriela R, Bertolini, Edson, Alfaro-Fernández, Ana, Martínez, Carmen, Tanaka, Francisco André Ossamu, Kitajima, Elliot W, Roselló, Montserrat, Sanjuán, Susana, Ferrándiz, Juan Carlos, López, María M, Cambra, Mariano, Font, María Isabel
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.08.2014
Schlagworte:
Apium graveolens - microbiology
Apium graveolens - ultrastructure
Base Sequence
Daucus carota - microbiology
DNA Primers - genetics
DNA, Plant - chemistry
DNA, Plant - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Haplotypes
Molecular Sequence Data
Phylogeny
Plant Diseases - microbiology
Plant Shoots - microbiology
Plant Shoots - ultrastructure
Plant Stems - microbiology
Plant Stems - ultrastructure
Polymorphism, Single Nucleotide - genetics
Real-Time Polymerase Chain Reaction - methods
Reproducibility of Results
Rhizobiaceae - genetics
Rhizobiaceae - isolation & purification
Rhizobiaceae - ultrastructure
Sequence Analysis, DNA
Spain
Species Specificity
Spain
ISSN:0031-949X
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Zusammenfassung:A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with 'Candidatus Liberibacter solanacearum' and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect 'Ca. L. solanacearum'. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of 'Ca. Liberibacter' were observed using electron microscopy in celery plant tissues. A fifth haplotype of 'Ca. L. solanacearum', named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.
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ISSN:0031-949X
DOI:10.1094/PHYTO-07-13-0182-R