Myonecrosis Induction by Intramuscular Injection of CTX

Skeletal muscle, one of the most abundant tissue in the body, is a highly regenerative tissue. Indeed, compared to other tissues that are not able to regenerate after injury, skeletal muscle can fully regenerate upon mechanically, chemically, and infection-induced trauma. Several injury models have...

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Vydáno v:Bio-protocol Ročník 13; číslo 1; s. e4587
Hlavní autoři: Feno, Simona, Munari, Fabio, Gherardi, Gaia, Vecellio Reane, Denis, D’Angelo, Donato, Viola, Antonella, Rizzuto, Rosario, Raffaello, Anna
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Bio-Protocol 05.01.2023
Bio-protocol LLC
Témata:
Biology
Methods
Cardiotoxin
Regeneration
Myonecrosis
Skeletal muscle injury
Skeletal muscle
ISSN:2331-8325, 2331-8325
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Shrnutí:Skeletal muscle, one of the most abundant tissue in the body, is a highly regenerative tissue. Indeed, compared to other tissues that are not able to regenerate after injury, skeletal muscle can fully regenerate upon mechanically, chemically, and infection-induced trauma. Several injury models have been developed to thoroughly investigate the physiological mechanisms regulating skeletal muscle regeneration. This protocol describes how to induce muscle regeneration by taking advantage of a cardiotoxin (CTX)-induced muscle injury model. The overall steps include CTX injection of tibialis anterior (TA) muscles of BL6N mice, collection of regenerating muscles at different time points after CTX injury, and histological characterization of regenerating muscles. Our protocol, compared with others such as those for freeze-induced injury models, avoids laceration or infections of the muscles since it involves neither surgery nor suture. In addition, our protocol is highly reproducible, since it causes homogenous myonecrosis of the whole muscle, and further reduces animal pain and stress. Graphical abstract.
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Contributed equally to this work
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.4587