Optimizing ex vivo penetration tests via quantitative confocal Raman spectroscopy: Impact of incubation time, skin hydration, surfactant treatment and UVA irradiation on caffeine distribution

[Display omitted] Ex vivo penetration tests are important tools in cosmetic and pharmaceutical research. However, variability of experimental setups is challenging when reviewing literature. Different skin models, pre-treatments and experimental parameters render comparison difficult. Thus, our aim...

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Vydáno v:International journal of pharmaceutics Ročník 667; číslo Pt B; s. 124932
Hlavní autoři: Steiner, Katja, Hübel, Pia, Srndic, Azra, Klang, Victoria
Médium: Journal Article
Jazyk:angličtina
Vydáno: Netherlands Elsevier B.V 25.12.2024
Témata:
Animals
Caffeine
Caffeine - pharmacokinetics
Confocal Raman spectroscopy
Humans
Penetration
Porcine ear skin
quantitative CRS
Skin - metabolism
Skin - radiation effects
Skin Absorption
Sodium Dodecyl Sulfate - analogs & derivatives
Sodium Dodecyl Sulfate - chemistry
Spectrum Analysis, Raman - methods
Surface-Active Agents - chemistry
Surfactant
Time Factors
Ultraviolet Rays
UVA
Water - chemistry
UVA
SLES
UV
CRS
quantitative CRS
Surfactant
SC
Porcine ear skin
Penetration
PC
PE
NE
Confocal Raman spectroscopy
LPC
PS80
Caffeine
HLB
MCT
ISSN:0378-5173, 1873-3476, 1873-3476
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Shrnutí:[Display omitted] Ex vivo penetration tests are important tools in cosmetic and pharmaceutical research. However, variability of experimental setups is challenging when reviewing literature. Different skin models, pre-treatments and experimental parameters render comparison difficult. Thus, our aim was to conduct ex vivo penetration tests using caffeine in different setups with varying incubation conditions (ambient vs. Franz cells, infinite vs. finite dose). Additionally, the impact of skin pre-treatment with different aggressors (surfactants, UVA irradiation) should be considered. Possible synergistic barrier damage of surfactants and UVA irradiation should be explored. Analysis was conducted using quantitative confocal Raman spectroscopy. Results showed that incubation time and extensive hydration (20 h in Franz cells) had the greatest impact on penetration behavior. Additional irradiation after pre-treatment with oil-in-water nanoemulsions showed no strong impact on caffeine penetration in general, irrespective of surfactant type. However, in case of sodium lauryl ether sulfate, a trend towards enhanced values was observed due to irradiation (1.3-fold). This suggests cumulative skin barrier damage of irritant surfactants and UVA irradiation, potentially due to stratum corneum alterations. Further studies using different irradiation regimens are planned to confirm this hypothesis.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0378-5173
1873-3476
1873-3476
DOI:10.1016/j.ijpharm.2024.124932