Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix

Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model...

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Veröffentlicht in:International journal of molecular sciences Jg. 24; H. 3; S. 2842
Hauptverfasser: Navaee, Fatemeh, Renaud, Philippe, Kleger, Alexander, Braschler, Thomas
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland MDPI AG 02.02.2023
MDPI
Schlagworte:
Animals
Cardiomyocytes
Cell Differentiation
Collagen
Collagen - analysis
Extracellular matrix
Extracellular Matrix - chemistry
Fibrin - analysis
Heart attacks
Hydrogels
Hydrogels - analysis
Mechanical properties
Myocytes, Cardiac
Rats
Stem cells
Swine
Thrombin
Tissue Engineering - methods
Tissue Scaffolds
H9c2 cell differentiation
beating synchrony
neonatal cardiomyocyte culture
3D co-culture
dECM-fibrin hydrogel
ISSN:1422-0067, 1661-6596, 1422-0067
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Zusammenfassung:Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency was achieved by 3D embedding of rat neonatal cardiomyocytes in the 3D system. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony, and the maintenance of spontaneous beating, as compared to various controls including Matrigel, pure fibrin and collagen I as well as a fibrin-collagen I blend.
Bibliographie:ObjectType-Article-1
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24032842