Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix
Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model...
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| Published in: | International journal of molecular sciences Vol. 24; no. 3; p. 2842 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
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| ISSN: | 1422-0067, 1661-6596, 1422-0067 |
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| Abstract | Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency was achieved by 3D embedding of rat neonatal cardiomyocytes in the 3D system. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony, and the maintenance of spontaneous beating, as compared to various controls including Matrigel, pure fibrin and collagen I as well as a fibrin-collagen I blend. |
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| AbstractList | Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency was achieved by 3D embedding of rat neonatal cardiomyocytes in the 3D system. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony, and the maintenance of spontaneous beating, as compared to various controls including Matrigel, pure fibrin and collagen I as well as a fibrin-collagen I blend. Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency was achieved by 3D embedding of rat neonatal cardiomyocytes in the 3D system. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony, and the maintenance of spontaneous beating, as compared to various controls including Matrigel, pure fibrin and collagen I as well as a fibrin-collagen I blend.Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employing an in vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin facilitates the gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. Our investigations revealed enhanced cardiogenic differentiation in the H9c2 myoblast cells when using the system in a co-culture with Nor-10 fibroblasts. Further enhancement of differentiation efficiency was achieved by 3D embedding of rat neonatal cardiomyocytes in the 3D system. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony, and the maintenance of spontaneous beating, as compared to various controls including Matrigel, pure fibrin and collagen I as well as a fibrin-collagen I blend. |
| Author | Navaee, Fatemeh Braschler, Thomas Kleger, Alexander Renaud, Philippe |
| AuthorAffiliation | 3 Institute of Molecular Oncology and Stem Cell Biology, Ulm University Hospital, 89081 Ulm, Germany 5 Organoid Core Facility, Medical Faculty, Ulm University Hospital, 89081 Ulm, Germany 2 Department of Pathology and Immunology, Faculty of Medicine, CMU, 1211 Geneva, Switzerland 1 Microsystems Laboratory-LMIS4, EPFL, 1015 Lausanne, Switzerland 4 Interdisciplinary Pancreatology, Department of Internal Medicine 1, Ulm University Hospital, 89081 Ulm, Germany |
| AuthorAffiliation_xml | – name: 1 Microsystems Laboratory-LMIS4, EPFL, 1015 Lausanne, Switzerland – name: 4 Interdisciplinary Pancreatology, Department of Internal Medicine 1, Ulm University Hospital, 89081 Ulm, Germany – name: 5 Organoid Core Facility, Medical Faculty, Ulm University Hospital, 89081 Ulm, Germany – name: 2 Department of Pathology and Immunology, Faculty of Medicine, CMU, 1211 Geneva, Switzerland – name: 3 Institute of Molecular Oncology and Stem Cell Biology, Ulm University Hospital, 89081 Ulm, Germany |
| Author_xml | – sequence: 1 givenname: Fatemeh surname: Navaee fullname: Navaee, Fatemeh – sequence: 2 givenname: Philippe surname: Renaud fullname: Renaud, Philippe – sequence: 3 givenname: Alexander orcidid: 0000-0003-0592-5232 surname: Kleger fullname: Kleger, Alexander – sequence: 4 givenname: Thomas surname: Braschler fullname: Braschler, Thomas |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36769166$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1002/cphy.c160006 10.1016/j.bbagen.2014.01.010 10.1021/acsami.6b14020 10.3109/15419069809010789 10.1529/biophysj.107.124545 10.1038/ncomms4935 10.1016/j.actbio.2018.05.056 10.1002/bit.22647 10.4137/ACI.S28905 10.1161/CIRCRESAHA.116.308139 10.1016/j.actbio.2014.11.035 10.1039/C3BM60283D 10.1371/journal.pone.0031756 10.1101/2020.01.30.927319 10.1093/humrep/dei286 10.1055/b-006-163285 10.1016/j.biomaterials.2013.11.021 10.1016/0014-4827(76)90447-X 10.1111/j.1538-7836.2004.00787.x 10.1038/srep03474 10.1161/CIRCRESAHA.114.301275 10.1371/journal.pone.0129303 10.1038/nmat4590 10.1089/ten.tea.2015.5000.abstracts 10.1007/s00253-013-4886-x 10.1002/(SICI)1096-8644(199804)105:4<539::AID-AJPA10>3.0.CO;2-1 10.1002/adfm.201300570 10.1089/ten.tea.2014.0591 10.1002/pmic.200900758 10.1529/biophysj.106.098574 10.1161/CIRCULATIONAHA.105.001297 10.1152/ajpheart.00023.2006 10.1016/S0142-9612(02)00230-2 10.1016/j.yjmcc.2016.03.005 10.1161/hh1101.091862 10.1038/am.2014.19 10.1002/adfm.201700427 10.1002/pola.10960 10.1002/adhm.201500236 10.1002/cphy.c140067 10.1016/j.actbio.2016.11.068 10.1016/j.stemcr.2015.02.007 10.1002/jbm.a.32951 10.1016/j.cossms.2016.02.001 10.1038/s41598-018-20307-2 10.1016/j.biomaterials.2009.06.045 10.1371/journal.pone.0062628 |
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| Keywords | H9c2 cell differentiation beating synchrony neonatal cardiomyocyte culture 3D co-culture dECM-fibrin hydrogel |
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| References | Feiner (ref_12) 2016; 15 Pati (ref_30) 2014; 5 ref_50 An (ref_1) 2015; 10 Huang (ref_42) 2006; 21 Jacot (ref_35) 2008; 95 Manso (ref_45) 2014; 114 ref_58 ref_57 ref_54 Schoen (ref_32) 2017; 27 Suhaeri (ref_39) 2016; 9 ref_52 Butler (ref_37) 2003; 41 Bick (ref_41) 1998; 6 ref_51 Kong (ref_26) 2013; 3 Singelyn (ref_55) 2009; 30 Bajaj (ref_5) 2010; 95A Ross (ref_47) 2001; 88 ref_61 Williams (ref_23) 2015; 14 ref_60 Annabi (ref_20) 2013; 23 Liu (ref_44) 2008; 94 ref_24 Bracaglia (ref_22) 2015; 4 Piacentini (ref_53) 2018; 76 ref_21 Cohen (ref_46) 2018; 8 ref_62 Canonico (ref_25) 2003; 74 ref_28 Baudino (ref_9) 2006; 291 Suhaeri (ref_29) 2015; 21 Annabi (ref_4) 2014; 6 Tallman (ref_59) 2004; 2 Agmon (ref_6) 2016; 20 Gattazzo (ref_16) 2014; 1840 Singelyn (ref_15) 2013; 5 ref_34 ref_33 Richardson (ref_3) 2015; 5 ref_31 Maddah (ref_63) 2015; 4 Streeter (ref_11) 2019; 1144 ref_38 Saldin (ref_19) 2017; 49 Cipitria (ref_18) 2015; 21 Hughes (ref_48) 2010; 10 Verhulsel (ref_17) 2014; 35 Johnson (ref_14) 2014; 2 Kalogeris (ref_2) 2016; 7 Kutschka (ref_13) 2006; 114 Zhou (ref_40) 2016; 118 Tio (ref_10) 2002; 23 Yang (ref_56) 1998; 105 Gundersen (ref_36) 2014; 98 ref_49 ref_8 Kimes (ref_27) 1976; 98 Bhana (ref_7) 2009; 105 Pellman (ref_43) 2016; 94 |
| References_xml | – volume: 7 start-page: 113 year: 2016 ident: ref_2 article-title: Ischemia/Reperfusion publication-title: Compr. Physiol. doi: 10.1002/cphy.c160006 – volume: 1840 start-page: 2506 year: 2014 ident: ref_16 article-title: Extracellular matrix: A dynamic microenvironment for stem cell niche publication-title: Biochim. Et Biophys. Acta (BBA) Gen. Subj. doi: 10.1016/j.bbagen.2014.01.010 – volume: 9 start-page: 224 year: 2016 ident: ref_39 article-title: Novel Platform of Cardiomyocyte Culture and Coculture via Fibroblast-Derived Matrix-Coupled Aligned Electrospun Nanofiber publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/acsami.6b14020 – ident: ref_49 – volume: 6 start-page: 301 year: 1998 ident: ref_41 article-title: Physical, contractile and calcium handling properties of neonatal cardiac myocytes cultured on different matrices publication-title: Cell Adhes. Commun. doi: 10.3109/15419069809010789 – ident: ref_51 – volume: 95 start-page: 3479 year: 2008 ident: ref_35 article-title: Substrate Stiffness Affects the Functional Maturation of Neonatal Rat Ventricular Myocytes publication-title: Biophys. J. doi: 10.1529/biophysj.107.124545 – volume: 5 start-page: 3935 year: 2014 ident: ref_30 article-title: Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink publication-title: Nat. Commun. doi: 10.1038/ncomms4935 – volume: 76 start-page: 71 year: 2018 ident: ref_53 article-title: Additive manufacturing of hierarchical injectable scaffolds for tissue engineering publication-title: Acta Biomater. doi: 10.1016/j.actbio.2018.05.056 – volume: 105 start-page: 1148 year: 2009 ident: ref_7 article-title: Influence of substrate stiffness on the phenotype of heart cells publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.22647 – volume: 10 start-page: 39 year: 2015 ident: ref_1 article-title: Organ-on-a-Chip: New Platform for Biological Analysis publication-title: Anal. Chem. Insights doi: 10.4137/ACI.S28905 – volume: 118 start-page: 368 year: 2016 ident: ref_40 article-title: Recounting Cardiac Cellular Composition publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.116.308139 – ident: ref_61 – volume: 14 start-page: 84 year: 2015 ident: ref_23 article-title: Cardiac extracellular matrix–fibrin hybrid scaffolds with tunable properties for cardiovascular tissue engineering publication-title: Acta Biomater. doi: 10.1016/j.actbio.2014.11.035 – volume: 2 start-page: 735 year: 2014 ident: ref_14 article-title: Human versus porcine tissue sourcing for an injectable myocardial matrix hydrogel publication-title: Biomater. Sci. doi: 10.1039/C3BM60283D – volume: 5 start-page: 173ra25 year: 2013 ident: ref_15 article-title: Safety and Efficacy of an Injectable Extracellular Matrix Hydrogel for Treating Myocardial Infarction publication-title: Sci. Transl. Med. – ident: ref_58 – ident: ref_8 – ident: ref_38 doi: 10.1371/journal.pone.0031756 – ident: ref_31 – ident: ref_54 doi: 10.1101/2020.01.30.927319 – ident: ref_62 – volume: 21 start-page: 202 year: 2006 ident: ref_42 article-title: Adverse effects of retinoic acid on embryo development and the selective expression of retinoic acid receptors in mouse blastocysts publication-title: Hum. Reprod. doi: 10.1093/humrep/dei286 – volume: 74 start-page: 21 year: 2003 ident: ref_25 article-title: The use of human fibrin glue in the surgical operations publication-title: Acta Bio-Med. Atenei Parm. – ident: ref_52 doi: 10.1055/b-006-163285 – volume: 35 start-page: 1816 year: 2014 ident: ref_17 article-title: A review of microfabrication and hydrogel engineering for micro-organs on chips publication-title: Biomaterials doi: 10.1016/j.biomaterials.2013.11.021 – volume: 98 start-page: 367 year: 1976 ident: ref_27 article-title: Properties of a clonal muscle cell line from rat heart publication-title: Exp. Cell Res. doi: 10.1016/0014-4827(76)90447-X – volume: 2 start-page: 1341 year: 2004 ident: ref_59 article-title: Effects of all-trans retinoic acid or chemotherapy on the molecular regulation of systemic blood coagulation and fibrinolysis in patients with acute promyelocytic leukemia publication-title: J. Thromb. Haemost. doi: 10.1111/j.1538-7836.2004.00787.x – volume: 3 start-page: 3474 year: 2013 ident: ref_26 article-title: Matrix identity and tractional forces influence indirect cardiac reprogramming publication-title: Sci. Rep. doi: 10.1038/srep03474 – ident: ref_24 – ident: ref_34 – volume: 114 start-page: 572 year: 2014 ident: ref_45 article-title: Integrins and Integrin-Associated Proteins in the Cardiac Myocyte publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.114.301275 – ident: ref_28 doi: 10.1371/journal.pone.0129303 – volume: 15 start-page: 679 year: 2016 ident: ref_12 article-title: Engineered hybrid cardiac patches with multifunctional electronics for online monitoring and regulation of tissue function publication-title: Nat. Mater. doi: 10.1038/nmat4590 – volume: 21 start-page: S-1 year: 2015 ident: ref_18 article-title: 2015 4thTERMIS World CongressBoston, Massachusetts September 8–11, 2015 publication-title: Tissue Eng. Part A doi: 10.1089/ten.tea.2015.5000.abstracts – volume: 98 start-page: 219 year: 2014 ident: ref_36 article-title: Microbial transglutaminase displays broad acyl-acceptor substrate specificity publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s00253-013-4886-x – volume: 105 start-page: 539 year: 1998 ident: ref_56 article-title: Improved DNA extraction from ancient bones using silica-based spin columns publication-title: Am. J. Phys. Anthropol. doi: 10.1002/(SICI)1096-8644(199804)105:4<539::AID-AJPA10>3.0.CO;2-1 – volume: 23 start-page: 4950 year: 2013 ident: ref_20 article-title: Highly Elastic Micropatterned Hydrogel for Engineering Functional Cardiac Tissue publication-title: Adv. Funct. Mater. doi: 10.1002/adfm.201300570 – volume: 21 start-page: 1940 year: 2015 ident: ref_29 article-title: Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment publication-title: Tissue Eng. Part A doi: 10.1089/ten.tea.2014.0591 – volume: 10 start-page: 1886 year: 2010 ident: ref_48 article-title: Matrigel: A complex protein mixture required for optimal growth of cell culture publication-title: Proteomics doi: 10.1002/pmic.200900758 – volume: 94 start-page: 1497 year: 2008 ident: ref_44 article-title: Role of Cyclic Strain Frequency in Regulating the Alignment of Vascular Smooth Muscle Cells In Vitro publication-title: Biophys. J. doi: 10.1529/biophysj.106.098574 – volume: 114 start-page: I-167 year: 2006 ident: ref_13 article-title: Collagen Matrices Enhance Survival of Transplanted Cardiomyoblasts and Contribute to Functional Improvement of Ischemic Rat Hearts publication-title: Circulation doi: 10.1161/CIRCULATIONAHA.105.001297 – volume: 291 start-page: H1015 year: 2006 ident: ref_9 article-title: Cardiac fibroblasts: Friend or foe? publication-title: Am. J. Physiol. Circ. Physiol. doi: 10.1152/ajpheart.00023.2006 – ident: ref_21 – volume: 23 start-page: 4793 year: 2002 ident: ref_10 article-title: Cardiac tissue engineering: Characteristics of in unison contracting two- and three-dimensional neonatal rat ventricle cell (co)-cultures publication-title: Biomaterials doi: 10.1016/S0142-9612(02)00230-2 – volume: 94 start-page: 22 year: 2016 ident: ref_43 article-title: Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: Mechanisms and model systems publication-title: J. Mol. Cell. Cardiol. doi: 10.1016/j.yjmcc.2016.03.005 – volume: 88 start-page: 1112 year: 2001 ident: ref_47 article-title: Integrins and the Myocardium publication-title: Circ. Res. doi: 10.1161/hh1101.091862 – volume: 6 start-page: e99 year: 2014 ident: ref_4 article-title: Hydrogels for cardiac tissue engineering publication-title: NPG Asia Mater. doi: 10.1038/am.2014.19 – volume: 27 start-page: 1700427 year: 2017 ident: ref_32 article-title: Electrospun Extracellular Matrix: Paving the Way to Tailor-Made Natural Scaffolds for Cardiac Tissue Regeneration publication-title: Adv. Funct. Mater. doi: 10.1002/adfm.201700427 – ident: ref_50 – volume: 41 start-page: 3941 year: 2003 ident: ref_37 article-title: Mechanism and kinetics of the crosslinking reaction between biopolymers containing primary amine groups and genipin publication-title: J. Polym. Sci. Part A Polym. Chem. doi: 10.1002/pola.10960 – volume: 4 start-page: 2475 year: 2015 ident: ref_22 article-title: Extracellular Matrix-Based Biohybrid Materials for Engineering Compliant, Matrix-Dense Tissues publication-title: Adv. Healthc. Mater. doi: 10.1002/adhm.201500236 – volume: 1144 start-page: 1 year: 2019 ident: ref_11 article-title: Therapeutic Cardiac Patches for Repairing the Myocardium publication-title: Adv. Exp. Med. Biol. – volume: 5 start-page: 1877 year: 2015 ident: ref_3 article-title: Physiological Implications of Myocardial Scar Structure publication-title: Compr. Physiol. doi: 10.1002/cphy.c140067 – volume: 49 start-page: 1 year: 2017 ident: ref_19 article-title: Extracellular matrix hydrogels from decellularized tissues: Structure and function publication-title: Acta Biomater. doi: 10.1016/j.actbio.2016.11.068 – volume: 4 start-page: 621 year: 2015 ident: ref_63 article-title: A Non-invasive Platform for Functional Characterization of Stem-Cell-Derived Cardiomyocytes with Applications in Cardiotoxicity Testing publication-title: Stem Cell Rep. doi: 10.1016/j.stemcr.2015.02.007 – volume: 95A start-page: 1261 year: 2010 ident: ref_5 article-title: Stiffness of the substrate influences the phenotype of embryonic chicken cardiac myocytes publication-title: J. Biomed. Mater. Res. Part A doi: 10.1002/jbm.a.32951 – ident: ref_60 – volume: 20 start-page: 193 year: 2016 ident: ref_6 article-title: Controlling stem cell behavior with decellularized extracellular matrix scaffolds publication-title: Curr. Opin. Solid State Mater. Sci. doi: 10.1016/j.cossms.2016.02.001 – volume: 8 start-page: 2237 year: 2018 ident: ref_46 article-title: Theory of frequency response of mechanically driven cardiomyocytes publication-title: Sci. Rep. doi: 10.1038/s41598-018-20307-2 – ident: ref_57 – volume: 30 start-page: 5409 year: 2009 ident: ref_55 article-title: Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering publication-title: Biomaterials doi: 10.1016/j.biomaterials.2009.06.045 – ident: ref_33 doi: 10.1371/journal.pone.0062628 |
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| SubjectTerms | Animals Cardiomyocytes Cell Differentiation Collagen Collagen - analysis Extracellular matrix Extracellular Matrix - chemistry Fibrin - analysis Heart attacks Hydrogels Hydrogels - analysis Mechanical properties Myocytes, Cardiac Rats Stem cells Swine Thrombin Tissue Engineering - methods Tissue Scaffolds |
| Title | Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix |
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