Obese Mice with Dyslipidemia Exhibit Meibomian Gland Hypertrophy and Alterations in Meibum Composition and Aqueous Tear Production

Background: Dyslipidemia may be linked to meibomian gland dysfunction (MGD) and altered meibum lipid composition. The purpose was to determine if plasma and meibum cholesteryl esters (CE), triglycerides (TG), ceramides (Cer) and sphingomyelins (SM) change in a mouse model of diet-induced obesity whe...

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Published in:International journal of molecular sciences Vol. 21; no. 22; p. 8772
Main Authors: Osae, Eugene A., Bullock, Tiffany, Chintapalati, Madhavi, Brodesser, Susanne, Hanlon, Samuel, Redfern, Rachel, Steven, Philipp, Smith, C. Wayne, Rumbaut, Rolando E., Burns, Alan R.
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 20.11.2020
MDPI
Subjects:
Adipose Tissue - chemistry
Adipose Tissue - metabolism
Animals
Ceramides - classification
Ceramides - isolation & purification
Ceramides - metabolism
Cholesterol Esters - classification
Cholesterol Esters - isolation & purification
Cholesterol Esters - metabolism
Diet, High-Fat - adverse effects
Dyslipidemias - etiology
Dyslipidemias - metabolism
Dyslipidemias - pathology
Epididymis - chemistry
Epididymis - metabolism
Fasting
Fatty acids
Humans
Hypertrophy - etiology
Hypertrophy - metabolism
Hypertrophy - pathology
Lipids
Male
Mass spectrometry
Meibomian Glands - metabolism
Meibomian Glands - pathology
Metabolic disorders
Mice
Mice, Inbred C57BL
Mice, Obese
Obesity
Obesity - etiology
Obesity - metabolism
Obesity - pathology
Plasma
Principal Component Analysis
Scientific imaging
Sphingomyelins - classification
Sphingomyelins - isolation & purification
Sphingomyelins - metabolism
Tears - chemistry
Tears - metabolism
Triglycerides
Triglycerides - classification
Triglycerides - isolation & purification
Triglycerides - metabolism
lipids
meibomian gland dysfunction
mass spectrometry
meibum
dry eye
dyslipidemia
obesity
ISSN:1422-0067, 1661-6596, 1422-0067
Online Access:Get full text
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Summary:Background: Dyslipidemia may be linked to meibomian gland dysfunction (MGD) and altered meibum lipid composition. The purpose was to determine if plasma and meibum cholesteryl esters (CE), triglycerides (TG), ceramides (Cer) and sphingomyelins (SM) change in a mouse model of diet-induced obesity where mice develop dyslipidemia. Methods: Male C57/BL6 mice (8/group, age = 6 wks) were fed a normal (ND; 15% kcal fat) or an obesogenic high-fat diet (HFD; 42% kcal fat) for 10 wks. Tear production was measured and meibography was performed. Body and epididymal adipose tissue (eAT) weights were determined. Nano-ESI-MS/MS and LC-ESI-MS/MS were used to detect CE, TG, Cer and SM species. Data were analyzed by principal component analysis, Pearson’s correlation and unpaired t-tests adjusted for multiple comparisons; significance set at p ≤ 0.05. Results: Compared to ND mice, HFD mice gained more weight and showed heavier eAT and dyslipidemia with higher levels of plasma CE, TG, Cer and SM. HFD mice had hypertrophic meibomian glands, increased levels of lipid species acylated by saturated fatty acids in plasma and meibum and excessive tear production. Conclusions: The majority of meibum lipid species with saturated fatty acids increased with HFD feeding with evidence of meibomian gland hypertrophy and excessive tearing. The dyslipidemia is associated with altered meibum composition, a key feature of MGD.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21228772