Obese Mice with Dyslipidemia Exhibit Meibomian Gland Hypertrophy and Alterations in Meibum Composition and Aqueous Tear Production

Background: Dyslipidemia may be linked to meibomian gland dysfunction (MGD) and altered meibum lipid composition. The purpose was to determine if plasma and meibum cholesteryl esters (CE), triglycerides (TG), ceramides (Cer) and sphingomyelins (SM) change in a mouse model of diet-induced obesity whe...

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Vydané v:	International journal of molecular sciences Ročník 21; číslo 22; s. 8772
Hlavní autori:	Osae, Eugene A., Bullock, Tiffany, Chintapalati, Madhavi, Brodesser, Susanne, Hanlon, Samuel, Redfern, Rachel, Steven, Philipp, Smith, C. Wayne, Rumbaut, Rolando E., Burns, Alan R.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Switzerland MDPI AG 20.11.2020 MDPI
Predmet:	Adipose Tissue - chemistry Adipose Tissue - metabolism Animals Ceramides - classification Ceramides - isolation & purification Ceramides - metabolism Cholesterol Esters - classification Cholesterol Esters - isolation & purification Cholesterol Esters - metabolism Diet, High-Fat - adverse effects Dyslipidemias - etiology Dyslipidemias - metabolism Dyslipidemias - pathology Epididymis - chemistry Epididymis - metabolism Fasting Fatty acids Humans Hypertrophy - etiology Hypertrophy - metabolism Hypertrophy - pathology Lipids Male Mass spectrometry Meibomian Glands - metabolism Meibomian Glands - pathology Metabolic disorders Mice Mice, Inbred C57BL Mice, Obese Obesity Obesity - etiology Obesity - metabolism Obesity - pathology Plasma Principal Component Analysis Scientific imaging Sphingomyelins - classification Sphingomyelins - isolation & purification Sphingomyelins - metabolism Tears - chemistry Tears - metabolism Triglycerides Triglycerides - classification Triglycerides - isolation & purification Triglycerides - metabolism lipids meibomian gland dysfunction mass spectrometry meibum dry eye dyslipidemia obesity
ISSN:	1422-0067, 1661-6596, 1422-0067
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Shrnutí:	Background: Dyslipidemia may be linked to meibomian gland dysfunction (MGD) and altered meibum lipid composition. The purpose was to determine if plasma and meibum cholesteryl esters (CE), triglycerides (TG), ceramides (Cer) and sphingomyelins (SM) change in a mouse model of diet-induced obesity where mice develop dyslipidemia. Methods: Male C57/BL6 mice (8/group, age = 6 wks) were fed a normal (ND; 15% kcal fat) or an obesogenic high-fat diet (HFD; 42% kcal fat) for 10 wks. Tear production was measured and meibography was performed. Body and epididymal adipose tissue (eAT) weights were determined. Nano-ESI-MS/MS and LC-ESI-MS/MS were used to detect CE, TG, Cer and SM species. Data were analyzed by principal component analysis, Pearson’s correlation and unpaired t-tests adjusted for multiple comparisons; significance set at p ≤ 0.05. Results: Compared to ND mice, HFD mice gained more weight and showed heavier eAT and dyslipidemia with higher levels of plasma CE, TG, Cer and SM. HFD mice had hypertrophic meibomian glands, increased levels of lipid species acylated by saturated fatty acids in plasma and meibum and excessive tear production. Conclusions: The majority of meibum lipid species with saturated fatty acids increased with HFD feeding with evidence of meibomian gland hypertrophy and excessive tearing. The dyslipidemia is associated with altered meibum composition, a key feature of MGD.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms21228772