HTSeq—a Python framework to work with high-throughput sequencing data

Motivation: A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard workflows, custom scripts are needed. Results: We present HTSeq, a Python library to facilitate the rapid development of such sc...

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Vydáno v:Bioinformatics (Oxford, England) Ročník 31; číslo 2; s. 166 - 169
Hlavní autoři: Anders, Simon, Pyl, Paul Theodor, Huber, Wolfgang
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Oxford University Press 15.01.2015
Témata:
Gene Expression Regulation
Genome, Human
Genomics - methods
High-Throughput Nucleotide Sequencing - methods
Humans
Original Papers
Software
ISSN:1367-4803, 1367-4811, 1367-4811
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Shrnutí:Motivation: A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard workflows, custom scripts are needed. Results: We present HTSeq, a Python library to facilitate the rapid development of such scripts. HTSeq offers parsers for many common data formats in HTS projects, as well as classes to represent data, such as genomic coordinates, sequences, sequencing reads, alignments, gene model information and variant calls, and provides data structures that allow for querying via genomic coordinates. We also present htseq-count, a tool developed with HTSeq that preprocesses RNA-Seq data for differential expression analysis by counting the overlap of reads with genes. Availability and implementation: HTSeq is released as an open-source software under the GNU General Public Licence and available from http://www-huber.embl.de/HTSeq or from the Python Package Index at https://pypi.python.org/pypi/HTSeq . Contact:  sanders@fs.tum.de
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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Associate Editor: Michael Brudno
ISSN:1367-4803
1367-4811
1367-4811
DOI:10.1093/bioinformatics/btu638