A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Production

The methylotrophic yeast Pichia pastoris is garnering interest as a chassis cell factory for the manufacture of recombinant proteins because it effectively satisfies the requirements of both laboratory and industrial set up. The optimisation of P. pastoris cultivation is still necessary due to strai...

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Vydáno v:	Molecular biotechnology Ročník 66; číslo 7; s. 1621 - 1639
Hlavní autoři:	Vijayakumar, Vijay Elakkya, Venkataraman, Krishnan
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	New York Springer US 01.07.2024 Springer Nature B.V
Témata:	alternative hosts Biochemistry Biological Products - metabolism Biological Techniques Biotechnology Cell Biology Chemistry Chemistry and Materials Science CRISPR CRISPR-Cas Systems Gene dosage Genetic diversity Genetic engineering Genetic Engineering - methods Human Genetics Komagataella pastoris manufacturing Methanol peptides Pichia Pichia - genetics Pichia - metabolism Pichia pastoris Process engineering Protein engineering Protein Science Proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Review Saccharomycetales - genetics Saccharomycetales - metabolism Systematic review therapeutics Yeast yeasts 2A peptide system Proteolysis Secretion Recombinant proteins CRISPR/Cas Promoters Codon optimization Pichia pastoris
ISSN:	1073-6085, 1559-0305, 1559-0305
On-line přístup:	Získat plný text
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Shrnutí:	The methylotrophic yeast Pichia pastoris is garnering interest as a chassis cell factory for the manufacture of recombinant proteins because it effectively satisfies the requirements of both laboratory and industrial set up. The optimisation of P. pastoris cultivation is still necessary due to strain- and product-specific problems such as promoter strength, methanol utilisation type, and culturing conditions to realize the high yields of heterologous protein(s) of interest. Techniques integrating genetic and process engineering have been used to overcome these problems. Insight into the Pichia as an expression system utilizing MUT pathway and the development of methanol free systems are highlighted in this systematic review. Recent developments in the improved production of proteins in P. pastoris by (i) diverse genetic engineering such as codon optimization and gene dosage; (ii) cultivating tactics including co-expression of chaperones; (iii) advances in the use of the 2A peptide system, and (iv) CRISPR/Cas technologies are widely discussed. We believe that by combining these strategies, P. pastoris will become a formidable platform for the production of high value therapeutic proteins. Graphical Abstract
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Undefined-3
ISSN:	1073-6085 1559-0305 1559-0305
DOI:	10.1007/s12033-023-00803-1