Unraveling the camel rumen microbiome through metaculturomics approach for agriculture waste hydrolytic potential

Cellulose is the most abundant natural polymer present on Earth in the form of agriculture waste. Hydrolysis of agriculture waste for simple fermentable reducing sugars is the bottleneck in the area of biofuel generation and other value-added products. The present study aims to utilize the camel rum...

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Vydané v:	Archives of microbiology Ročník 203; číslo 1; s. 107 - 123
Hlavní autori:	Srivastava, Shweta, Dafale, Nishant A., Jakhesara, Subhash J., Joshi, Chaitanya G., Patil, Niteen V., Purohit, Hemant J.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2021 Springer Nature B.V
Predmet:	Agricultural wastes Agriculture Animals Bacillus subtilis Bacteria Bacteria - classification Bacteria - genetics Bacteria - metabolism Biochemistry Biofuels Biofuels - microbiology Biomedical and Life Sciences biopolymers bioprocessing Bioreactors Biotechnology camels Camelus - microbiology Cell Biology Cellulase - metabolism Cellulose Cellulose - metabolism Citrobacter Crop residues Ecology endo-1,4-beta-glucanase Endoglucanase Enterobacter Enzymes Hydrolysis Life Sciences Microbial Ecology Microbiology microbiome Microbiomes Microbiota Natural polymers Original Paper Paenibacillus Polymers Pseudomonas Random Amplified Polymorphic DNA Technique Residues RNA, Ribosomal, 16S - genetics rRNA 16S Rumen Rumen - microbiology Screening Substrates Sugar value added Metaculturomics Microbial diversity Agriculture waste hydrolysis Enzyme activity Camel microbiome
ISSN:	0302-8933, 1432-072X, 1432-072X
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Shrnutí:	Cellulose is the most abundant natural polymer present on Earth in the form of agriculture waste. Hydrolysis of agriculture waste for simple fermentable reducing sugars is the bottleneck in the area of biofuel generation and other value-added products. The present study aims to utilize the camel rumen as a bioreactor for potent cellulolytic and hemicellulolytic bacteria by altering the feed types with varying cellulosic concentrations. A total of 6716 bacterial cultures were subjected to three layers of screening, where plate zymography and chromophoric substrate screening served as primary screening method for cellulolytic and hemicellulolytic potential. The potential isolates were genetically grouped using RAPD, and 51 representative isolates from each group were subjected to molecular identification through 16S rDNA sequencing, followed by quantification of various cellulolytic and hemicellulolytic enzymes. Out of 51 potent isolates, 5 isolates had high endoglucanase activity ranging from 0.3 to 0.48 U/ml. The selected five key isolates identified as Pseudomonas, Paenibacillus, Citrobacter, Bacillus subtilis, and Enterobacter were employed for hydrolyzing the various agriculture residues and resulted in approximately 0.4 mg/ml of reducing sugar. Furthermore, the metaculturomics approach was implemented to deduce the total cultured diversity through 16S rRNA amplicon library sequencing. The metaculturomics data revealed the dominance of proteobacteria and unidentified bacterial population in all four feed types, which indicates the possibility of culturing novel cellulose-deconstructing bacteria. Moreover, the presence of diverse hydrolytic enzymes in cultured isolates supports the usage of these bacteria in bio-processing of agriculture waste residues and obtaining the biofuels and other value-added products.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0302-8933 1432-072X 1432-072X
DOI:	10.1007/s00203-020-02010-x