Characterization of attenuated Renibacterium salmoninarum strains and their use as live vaccines

Two nutritionally mutant strains of Renibacterium salmoninarum (Rs) were isolated that grew on tryticase soy agar (Rs TSA1) or brain heart infusion agar (Rs BHI1). These 2 strains could be continuously cultured on these media, whereas typical R. salmoninarum would only grow on KDM-2 agar. We determi...

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Bibliographic Details
Published in:Diseases of aquatic organisms Vol. 44; no. 2; p. 121
Main Authors: Daly, J G, Griffiths, S G, Kew, A K, Moore, A R, Olivier, G
Format: Journal Article
Language:English
Published: Germany 09.03.2001
Subjects:
Animals
Bacterial Vaccines
Fish Diseases - prevention & control
Gram-Positive Bacterial Infections - prevention & control
Gram-Positive Bacterial Infections - veterinary
Micrococcaceae - immunology
Salmo salar
Vaccination - veterinary
Vaccines, Attenuated
ISSN:0177-5103
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Summary:Two nutritionally mutant strains of Renibacterium salmoninarum (Rs) were isolated that grew on tryticase soy agar (Rs TSA1) or brain heart infusion agar (Rs BHI1). These 2 strains could be continuously cultured on these media, whereas typical R. salmoninarum would only grow on KDM-2 agar. We determined no other phenotypic difference that could be used to distinguish them from wild-type R. salmoninarum. Both strains were found to be avirulent when 5 x 10(6) bacteria were intraperitoneally (i.p.) injected into Atlantic salmon. Rs TSA1, Rs BHI1, and Rs MT-239 (a R. salmoninarum strain previously shown to be attenuated) were tested as live vaccines in 2 separate trials. The best protection was seen with Rs TSA1. Vaccinated Atlantic salmon had relative percent survival (RPS) of 50 at 74 d post-challenge in Trial 1 and 76 at 60 d post-challenge in Trial 2. In both trials, 100% of the control salmon died from bacterial kidney disease (BKD) (within 40 d for Trial 1 and 50 d for Trial 2) after i.p. challenge with 5 x 10(6) live cells of the virulent isolate Rs Margaree.
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ISSN:0177-5103
DOI:10.3354/dao044121