The effects of staphylococcal protein A on human lymphokine-activated killer cell induction

Staphylococcal protein A (Cowan strain; SpA), a biologically active molecule capable of inducing augmented natural killer (NK) cell cytotoxicity, was studied in regard to its effects on lymphokine-activated killer (LAK) cell development. SpA, when co-cultured with interleukin-2 (IL-2) for 4 days, si...

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Published in:Cancer immunology, immunotherapy : CII Vol. 33; no. 2; p. 97
Main Authors: Lindemann, R A, Singh, K P, Shau, H, Gupta, R K
Format: Journal Article
Language:English
Published: Germany 01.03.1991
Subjects:
Antigens, Differentiation, T-Lymphocyte - analysis
CD56 Antigen
Drug Synergism
Flow Cytometry
Humans
Immunoglobulin G - physiology
Interferon-gamma - physiology
Interleukin-2 - pharmacology
Killer Cells, Lymphokine-Activated - drug effects
Killer Cells, Lymphokine-Activated - immunology
Staphylococcal Protein A - pharmacology
ISSN:0340-7004
Online Access:Get more information
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Summary:Staphylococcal protein A (Cowan strain; SpA), a biologically active molecule capable of inducing augmented natural killer (NK) cell cytotoxicity, was studied in regard to its effects on lymphokine-activated killer (LAK) cell development. SpA, when co-cultured with interleukin-2 (IL-2) for 4 days, significantly augmented both LAK activity against NK-resistant M14 (melanoma) target cells and DNA synthesis of peripheral blood mononuclear cells (PBMC). This enhancement occurred with SpA concentrations of 1-100 micrograms/ml in a dose-dependent fashion; concentrations above 100 micrograms/ml were no more effective. When SpA (10 micrograms/ml) was added to PBMC cultures with various IL-2 concentrations, cytotoxicity was increased over controls with IL-2 alone. The peak cytotoxic effect reached a plateau at 80 U/ml IL-2. SpA alone induced early (day 1) cytotoxicity, which rapidly declined. SpA alone did not induce PBMC proliferation but it did increase expression of CD25 (Tac), IL-2 receptor alpha chain, on CD56(Leu19)-positive and -negative cells. The potentiating effect of SpA was significantly enhanced in serum-free medium. If either human AB serum or human IgG was added to cultures SpA-enhanced LAK cytotoxicity was diminished. The addition of anti-interferon gamma (anti-IFN gamma) antibody, but not anti-IFN alpha, inhibited (SpA+IL-2)-induced cytotoxicity, indicating that IFN gamma is partially responsible for the additive cytotoxic effect.
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ISSN:0340-7004
DOI:10.1007/BF01742536