Arabinogalactan from edible jambo fruit induces different responses on cytokine secretion by THP-1 macrophages in the absence and presence of proinflammatory stimulus

Syzygium jambos is an Indo-Malaian found in many tropical countries and it is mainly composed of carbohydrates. Fraction PF-WSP and SF-WSP were obtained by aqueous extraction followed by Fehling’s treatment. Monosaccharide analysis showed that fraction PF-WSP has a high content of uronic acids (90%)...

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Veröffentlicht in:International journal of biological macromolecules Jg. 107; H. Pt A; S. 35 - 41
Hauptverfasser: Tamiello, Camila Silva, do Nascimento, Georgia Erdmann, Iacomini, Marcello, Cordeiro, Lucimara M.C.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 01.02.2018
Schlagworte:
arabinogalactans
arabinose
Cytokine secrection
galactose
galacturonic acid
inflammation
interleukin-10
interleukin-1beta
Jambo type II arabinogalactan
macrophages
methylation
nuclear magnetic resonance spectroscopy
secretion
Syzygium jambos
THP-1 cells
tumor necrosis factor-alpha
viability
Jambo type II arabinogalactan
THP-1 cells
Cytokine secrection
ISSN:0141-8130, 1879-0003, 1879-0003
Online-Zugang:Volltext
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Zusammenfassung:Syzygium jambos is an Indo-Malaian found in many tropical countries and it is mainly composed of carbohydrates. Fraction PF-WSP and SF-WSP were obtained by aqueous extraction followed by Fehling’s treatment. Monosaccharide analysis showed that fraction PF-WSP has a high content of uronic acids (90%) and fraction SF-WSP presented mainly galactose (39.1%) and arabinose (34.2%), as neutral sugars and 9% of galacturonic acid. The presence of type II arabinogalactan in SF-WSP was evidenced by methylation analysis and 13C/1H HSQC NMR experiments. Immunomodulating properties of SF-WSP was investigated. It decreases THP-1 macrophage viability at the highest concentration tested (200μg/mL). We then tested non-cytotoxic concentrations of SF-WSP on THP-1 cytokine production in the presence and absence of LPS. The results showed that SF-WSP increased TNF-α, IL-1β and IL-10 secretion in a concentration-dependent manner as well as attenuated the inflammatory response induced by LPS at the highest concentration (100μg/mL). These results contribute to elucidate the effects of fruit pectic polysaccharides on immune cells.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0141-8130
1879-0003
1879-0003
DOI:10.1016/j.ijbiomac.2017.08.148